A Study On The Expression Of Heat Shock Protein 10 During Genesis And Development Of Large Bowel Carcinoma

Heat shock proteins (HSPs) are a group of highly conserved proteins during phylogenesis that reside extensively in both prokaryotic and eukaryotic cells and increase remarkably under physiological stress or pathologic state, and are vital to the survival of organism under brutal conditions. HSPs mainly act as chaperonins which involve in protein synthesizing and folding. In recent years, a number of studies have reported that they played a role in cancer biological behavior, tumor immunity, drug resistance, apoptosis, oncotherapy and prognosis.Heat shock protein 10 (HSP10) is a kind of micromolecular chaperonins that reside in mitochondrial and cytoplasm, mainly acting as chaperonins in mitochondrial protein folding process. The following two factors indicate that a close relationship may exist between HSP10 and tumors. (1) Early pregnancy factor (EPF). EPF was first described as immunity suppression factor and growth factor that appeared in serum of pregnant women during their early trimester and was necessary in early embryonic development. Subsequent studies demonstrated that EPF could be detected in serum of human malignant tumors, including bladder carcinoma, melanoma, chorioepithelioma and metastasizing mole.EPF has been identified as an extracellular homologue of HSP10 since its amino acid sequence and molecular weight are similar to HSP10. (2) Heat shock protein 60 (HSP60) . HSP60 and HSP10 are both mitochondrial chaperonins. Genes of HSP60 and HSP10 are localized head to head on chromosome 2 position q33.1 separated by a bi-directional promoter. HSP60 and HSP10 are closely related to each other in their functions and structures. A number of studies have reported that HSP60 had close relationship with various carcinomas, including bronchial carcinoma, colorectal adenocarcinoma, breast cancer and ovarian cancer.In the present study, we detected the expression of HSP10 in normal colonic mucosa, colorectal adenomas and adenocarcinomas by immunohistochemistry EnVision^TM and polymerase chain reaction(PCR). We analysed differential expression of HSP10 during development of large bowel carcinomas, among different degrees of displasia in colorectal...