NGF,Bcl-2,Bax Gene Expression In Contusive And Lacerated Brain Tissue Of Different Age Patients.

In clinical studies, it is found that the outcome of traumatic brain injury(TBI) appears to be worsen with increasing age, elderly patients would experience much more neurological deficits, higher cognitive morbidity, slower recovery rates, and higher mortality rates than young patients. In 2000 years, Clark demonstrated that the increase of Bcl-2 gene expression might be an important reason of higher recovery rates in children after TBI. In 2003 years, Gao Jin Xi demonstrated that the expression of NGF, Bcl-2 and Bax protein after fluid percussion brain injury in rat was influenced by aging, the low level of NGF expression and the decrease of Bcl-2/Bax ratio maybe play an important role in the age-related neurological deficits after TBI. We observed brain injury in an effort to elucidate the pathophysiology of this sensitivity, and in particular to determined if there are susceptibility differences in the expression of nerve growth factor(NGF), Bcl-2 and Bax gene in the contusive and lacerated brain tissue of immature, mature and aged patients.Objective: To study the effect of age on the expression of NGF, Bcl-2 and Bax protein after traumatic brain injury(TBI), probing into the molecule biology mechanism of the effect that age brings to the prognosis of TBI patient. Methods: The expression of NGF, Bcl-2 and Bax protein during 3 hours to 9 hours in contusive and lacerated brain tissue of aged group (≥ 60 years), mature group(19 years～59 years) and immature group (≤ 18 years) was analyzed by immunohistochemistry and digital image analysis system. Results: In the contusive and lacerated brain tissue, the expression of NGF, Bcl-2 and Bax protein was increased after TBI in immature , mature and aged brain tissue. However, the level of NGF protein and the ratio of Bcl-2/Bax were obviously lower in aged contusive and lacerated brain tissue compared to that of the immature and mature (P<0.05); the level of NGF protein and the ratio of Bcl-2/Bax were obviously lower in mature contusive and lacerated brain tissue compared to that of the immature (P<0.05). Conclusion: Bcl-2 and Bax gene is involved in delayed neuron death after TBI, and the turnover of neuron is influenced by the ratio of Bcl-2/Bax after TBI. The study demonstrated that the expression of NGF, Bcl-2 and Bax protein after TBI was...