Influence Of Cardiopulmonary Bypass On Lymphocyte Subsets And TIMP Of Patients

Objective: To evaluate the effects of cardiopulmonary bypass on cellular immune function of organism by detecting the change of peripheral blood lymphocyte subsets count, elucidate the significance of the change.Methods: Six patients undergoing cardiac surgery with cardiopulmonary bypass(CPB) of Shandong university Qilu hospital, five male and one female. Age 55.5±13 years, weight 67.4±7.2kg, cardiac functional grading is second or third grade. A fixed dose of heparin(3mg/kg) was administered to all patients before cardiopulmonary bypass(CPB). Activated clotting time(ACT) must longer than 480 seconds during cardiopulmonary bypass. At the end of cardiopulmonary bypass protamine was given to neutralize heparin. The protamine dose was determined by the heparin dose. All patients undergoing surgery under intravenous and inhalation combined anesthesia, were operated with a bubble oxygenater and controled the circulating temperature at moderate hypothermia(28～32℃). Aortic root infusion with cold crystal cardioplegic solution after clamping the ascending aorta. The samples of peripheral artery blood were drawn from the radial artery catheter immediately pre-operatively (T0) , the end of cardiopulmonary bypass(T1) and 12 hours after the end of the operation(T2). Blood samples were studied for lymphocyte subpopulations with flow cytometry.Results: Phenotypic analysis of peripheral blood lymphocytes was performed before the operation and immediately after cardiopulmonary bypass and 12 hours after...