The Study On Relationship Between Calcitonin Receptor Gene Polymorphism And Bone Mineral Density In Anhui Postmenopausal Women

Objective Osteoporosis is one of the most common disorders of aging. Osteoporosis is a bone disease characterized by low bone mass, decreasing bone mineral density(BMD). Researches have shown that osteoporosis is a polygenic disease, and the peak bone mass and bone mass loss are significantly determined by genetics factors. One of the genes involved in this thesis is the calcitonin receptor(CTR) gene. In recent years, the relationship between allelic variants of human calcitonin receptor and population was reported. However, the results were different in different population. The objective was to explore the relationship between polymorphism of the calcitonin receptor gene and BMD in postmenopausal women in Anhui. Methods The CTR genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) in 240 Anhui postmenopausal women and the distribution of CTR genotypes were calculated. BMD of lumbar, femur neck, Ward's and GT were measured by dual-energy X-ray absorptiometry. The relationship between BMD and polymorphism of CTR gene was analyzed. Meanwhile, we investigated the relationship of years since menopause, age of menopause, age of menarche and BMDof Anhui postmenopausal women. Results The PCR product was digested by Alu- â… , resulting in 228bp,120bp,108bp. The genotype of CTR was determined by polypropylene electrophoresis. Only CC and TC genotypes were observed. The distribution of CTR genotypes in 240 Anhui women was CC genotype 95.8%, TC genotype 4.2%, TT genotype 0%, respectively. The frequency of Allele C was 0.979 and that of allele T was 0.021. No significant differernce was found in the associations of two genotypes and...