| The anti-hepatic cirrhosis effects of BJ-JN and its mechanisms of action were studied. The main contents are as follows:1. Effect of BJ-JN on bile duct ligation (BDL)- induced cholestasis cirrhosis in ratsThe cholestasis cirrhosis models in rats were induced by BDL to investigate the effects of BJ-JN on anti-hepatic cirrhosis and improving blood circulation. The results showed that not only the elevated indices of liver and spleen, the elevated level of ALT in plasma, the content of HyP, MDA and ascites of rats were significantly reduced, but also the depressed activity of SOD and TP of liver homogenates and the content of potassium and sodium excreted from urine for 24 hours were obviously increased by BJ-JN (3, 6, 12g·kg-1) treatment. The elevated viscosities of whole blood either mPas3 and mPas4 was decreased significantly by 3 g/kg of BJ-JN or mPasl by 6 g/kg of BJ-JN. The shear rates elevated after BDL, including 1/200 s, 1/30 s, 1/5 s, 1/1 s, were decreased significantly by BJ-JN (12 g·kg-1). The elevated plasma viscosity (shear rate l/100s) was also decreased significantly by BJ-JN (6, 12 g·kg-1). Histopathology evaluation showed that the infiltration of inflammatory cells and the injury of hepatic cells were significantly decreased by BJ-JN (3, 6, 12 g·kg-1) the degree of liver cirrhosis induced by BDL was obviously alleviated by BJ-JN (12 g·kg-1), and the degree of liver fibrosis and the hyperplasia of bile duct cell. The results of immunohistochemical staining also showed that the expression of hepatocyte pSmad3 protein in rats induced by BDL was decreased by BJ-JN (3, 6, 12 g·kg-1) treatment. |
PDF Full Text Request