Expression Of VEGF/VEGFR In Human Hepatoma Cell Lines And Its Role In The Growth Of The Cells In Vivo

Objective: The biological feature of solid tumors determines their vascellum-dependency. In 1971 Folkman suggested the opinion that cancer could be inhibited through anti-angiogenic therapy. As the key factor in angiogenesis, VEGF (vascular endothelial growth factor, VEGF) has been widely studied in anti-angiogenic therapy. The recent studys found the spetific receptors of VEGF (VEGFR1, FLT-1; VEGFR2, KDR) also exit in the tumor cells, which indicated that VEGF acted in an autocrine manner in some tumor cell lines and some tumors. VEGF plays a key role in hepatoma, which is a high vascular-density tumor. There were few studies about the expression and significence of VEGF/VEGFR in human hepatoma cell lines. The aim of this study was to investigate the expression of VEGF/VEGFR in human hepatoma cell lines, screen the human hepatoma cell lines of VEGF of high-expressed and low-expressed, discuss the relation between VEGF and the growth of the cells in vivo, so as to provide the rationale for further exploration, and investigate the expression and significance of VEGF receptors in human hepatoma cells.Methods: ELISA was used to detect the VEGF in cell culture fluid, Western Blot was used to detect the VEGF in cell plasma. Cell lines of VEGF high-expressed and low-expressed were screened and were injected...