Epicutanous Sensitization With Ovalbumin Or Staphylococcal Super Antigen On Tape-stripped BALB/c Mice Skin To Induce And Allergic Skin Inflammation

Objectives:Previous studies showed the skin, after repeated epicutaneous ovalbumin(OVA) exposure induced a predominant Th2-like response with high IgE production in BALB/c mice. What's more, disruption of the stratum corneum allows potent epicutaneous immunization with protein antigens resuLting in a dominant systemic Th2 response, which is the feature of atopic dermatitis(AD) and protein contact dermatitis. Based on these, we sought to determine whether epicutaneous sensitization of tape-stripped BALB/c mice with OVA resuLts in allergic skin inflammation, whether elevated serum level of total IgE and Th2-bias cytokine mRNA expression wo μ Ld be induced. Staphylococcal enterotoxins have been implicated in the exacerbations of the inflammatory skin lesions in the compromised skin in atopic dermatitis. We sought to determine with the same sensitization protocol as OVA, Staphylococcal enterotoxin A (SEA) or Staphylococcal enterotoxin B (SEB) wo u Ld induce an allergic skin inflammation which shows features in AD. Methods...