Orthotopic Grafts Of Human Gliomas Into Nude Mice And Its MRI Research

Part one : Preliminary study on MR images of Balb/c mouseintracranial implantsObjective:To investigate whether 1.5T MRI can be utilized to perform imaging research inmouse bearing intracranial transplantation.Methods:Two medium implantations( Gadopentetate Meglumine and MethylthioniniumChloride) were injected at different dose into the right frontal hemisphere of all Balb/cmice under the guidance of stereotaxic apparatus. 1.5T magnetic resonance imaging(MRI)scans were performed in all animals to detect and measure implantation. At last, all miceinjected with Methylthioninium Chloride were killed to observe the location ofimplantation.Results:1.5T MRI can provide high quality images of the mouse head, as well asimplantation. The volume of the implantation could be figured out conveniently via 3Dscanning.Conclusion:The improved 1.5T MRI could precisely target and measure implantation inmouse brain. In the extended work, it can be applied to imaging research in nude mousemodel bearing intracranial glioma.Part two : Orthotopic grafts of human gliomas into nude micePurpose: To improve human glioma xenografts implanted intracranial in nude mice bytumor tissue injected.Methods: NC nude mice was injected subcutaneouly with SHG44 glioma cells(10~7),when we got human glioblastoma xenograft model, then implanted intracranial in nudemice quantified with the xenograft tumor tissue .4 weeks later ,all the mice weresacrificed.Slices of intracerebral tumor transplants was stained with hematein-eosin(HE).We study it's histologic characteristics under microscope.Results:3 of the 18 orthotopic grafted nude mice was died earlier , one of them did't formtumor. 30 days after tumor tissue implanted, all the mice were sacrificed. The brian was cut...