Construction And Instantaneous Expression Of Mouse SOX1 Eukaryotic Fluorescent Expression Vector

BackgroundRecently, stem cell transplantation has gained more and more interest in medical field for its use in the treatment of central nervous system diseases such as trauma and degenerative disease. The stem cells for cell transplantation mainly include embryonic stem cells ,neural stem cells and bone mesenchymal stem cells(MSCs) .In the three types of cells, MSCs can be easily obtained from bone marrow and are relatively easy to isolate and culture; MSCs transplantation has also no problems such as ethic and immune rejection , Thus, MSCs have the potential to provide an efficient and renewable source of cells for autologous transplantation .Mesenchymal stem cell (MSC) derived from the bone marrow are multipotent stem cells with the capacity to differentiate,under specific experimental conditions, into several different types of cells including osteoblasts, adipocytes, chondrocytes, skeletal muscle fibers. It has recently been reported that bone marrow-derived cells have also the potential to develop into neural lineages, such as neurons and astrocytes, both in vivo and in vitro. However the clinical use of MSCs in future is restricted for the low differentiation rate and unstability of the MSCs-derived neuron-like cells.To find a way by which MSCs can be induced into neural cells more efficienly and steadily will greatly promote the clinical use of MSCs in neural transplantation field.