Construction And Transient Expression Of The Eukaryotic Expression Vector Of Human Growth Hormone

Human growth hormone is secreted episodically by the anterior pituitary somatotrophs. It stimulates growth and development, regulates substance metabolism and immune function. The action of GH is numerous and affects almost every tissue and organ, especially the bone, muscle and internal organ. The human growth hormone gene spans 26kb on chromosome17q, which contains five exons, four introns and 3',5' untranslated region. Human growth hormone gene is translated to prohormone, which has 217 amino acids. Prohormone turns into human growth hormone by the effect of enzymolysis. As a hydrophilic globulin, human growth hormone contains 191 amino acids and possesses a molecular mass of approximately 22,000 daltons.Recently, it has become clear that growth hormone (GH) deficiency is by far more frequent disease than it was previously thought. Adult GH deficiency is a distinct nosological entity, with effects on cardiovascular risk factors(hyperlipidaemia, decreased fibrinolysis, increased atherosclerosis), abnormalities in cardiac structure and function, abnormalities in body composition(decrease lean body mass, increase body fat, reduce extracelluar water), reduced exercise performance and decreased areal bone mineral density; GH deficiency in children leads to dwarfism. In a word, GH deficiency associates with remarkable morbidity and mortality.Before recombinant DNA technology was developed, human growth hormone was obtained in limited supply from the pituitary glands of human cadavers since...