Analysis On The CD4～+CD25～(high) Regulatory T Cells In Peripheral Blood Of Chronic HBV Infected Patients

Chronic hepatitis B virus (HBV) infection has been a serious health problem for human beings. Due to the lack of effective treatment, every year about 1 million people die from severe hepatitis, liver cirrhosis, hepatocellular carcinoma caused by chromc HBV infection. Until now it is unclear why an individual develops a chronic carrier state. However, an inadequate immune response of the host is thought to play a critical role in the chronicity of the infection.CD4⁺CD25^high regulatory T cell study is one of the hot topics in immunology in the past few years. These cells suppress the autoimmune reactions and transplant immune reactions, playing an important role in stabilizing immune reactions and reducing immune injury. Meanwhile, they also suppress the anti-infection and anti-tumor immune reactions.The aim of this study was to analyze the amount of CD4⁺CD25^high regulatory T cells in the peripheral blood mononuclear cells of different clinical chronic HBV patients and its correlation with multiple clinical parameters.Methods and Materials:1. Analysis of CD4⁺CD25^high regulatory T cells in newborn infants cord blood and normal adult peripheral blood with flow cytometryCD4 CD8 CD3 and CD25 monoclonal antibodies labeled with fluorescence were used in immunofluorescence staining of the peripheral blood of normal adults...