Deer Antler Promotes Growth Of Ovariectomized Uterus And Up-regulates LIF And Le～Y Oligosaccharide Expression In Endometrium Of Mice

Objective:Recent study suggests that leukemia inhibitory factor (LIF),which has been regarded as a key cytokine, can promote uterus development and embryo implantation. In LIF-null mice, emryos develop to the blastocyst stage but do not implant. However, pregnancies do result in LIF-null mothers after infusion or injection of exogenous LIF, and LIF-null embryos can implant after transfer into wild-type pseudopregnant recipient uterus. In mice, the highest levels of LIF mRNA are found prior to implantation in glandular epithelium and luminal epithelium on the morning day 4 of pregnancy. Highest levels of LIF protein have also been reported on day 4, mainly in epithelium but some in stroma. In ovariectomized mice, uterine LIF mRNA increases markedly within 1 h of oestrogen injection and is not affected by progesterone, strongly suggesting oestrogenic control. LIF signal may be important in impantation.Le~Y antigen on surface cell is a fucosylated Lewis oligosaccharide,α1,2- fucosyltranferase (FUT1) andα1,3-fucosyltranferase (FUT4) are key enzymes of Le~Y antigen synthesis, The recent study of our lab found that Le~Y was regarded as a mediator to initiate the embryo attachment. Le~Y expression is controlled by estradiol in mice. Le~Y on the endometrium was expressed highly on the fourth day 4 at implantation, and endometria which exposed to specific antibody to Le~Y could be inhibited in the process of implantation. Moreover, Le~Y was expressed lowly in the endometria's women who were sterile. The above data suggested that this Le~Y antigen has a close relationship with implantation. Ovary hormones can regulate...