Effect Of Shikonin On Proliferation Of Bladder Cancer T24 Cells And The Mechanism

Objective To investigate proliferation inhibiting effect of shikonin on bladder cancer T24 Cells and its mechanism.Methods The bladder T24 cells were treated with shikonin in 25 μmol/L, 50μmol/L and 75 μmol/L for 12 hour, 24 hour or 48 hour. Cell Proliferation inhibition was determined with MTT.COX-2 mRNA exression level was assessed by reverse transcriptase polymerase chain reaction(RT-PCR), and exprssion of COX-2 protein was evaluated by western blot after the bladder T24 cells were treated with shikonin in 25 μmol/L, 50 μmol/L and 75 μmol/L for 24 hour or 48 hour.. At the same time, it was set up a control group (0μ mol/L) and a celecoxib group (50μ mol/L).Results 50 μmol/L celecoxib could inhibit proliferation of T24 cell , decreased expression of COX-2 mRNA and protein. Shikonin could significantly inhibit proliferation of T24 cell at concentrations from 25 to 75 μ mol/L. There were great statistical signification between varies medicated group and control group (P<0.05) ,and there also were great statistical signification between medicated group each other(P<0.05). Cells Proliferation inhibition rate increased in a dose and time-dependent manner and expression of COX-2 mRNA and protein was also decreased by various concentrations of shikonin in a dose and time -dependent manner.Conclusion Shikonin could significantly inhibit proliferation of T24 cell, COX-2 maybe involved in it.