Novel Detection Methods Of 8-Hydroxy-2'-deoxyguanosine As A Biomarker Of DNA Damage And Their Application

DNA adduct is a covalent complex formed by DNA and electrophilic activity production of toxicological chemicals transformed in body, and it is not only an exposure biomarker but also an effect biomarker. 8-Hydroxy-2'-deoxyguanosine is one of the most representative biomarkers of oxidative DNA damage. To develop novel detection method for 8-hydroxy-2'-deoxyguanosine has important medical significance.In the chapter 2, a new detection method has been developed with th technology of Resonance Light Scattering. It is found that the intensity of system's Resonance Light Scattering (RLS) is significantly enhanced duo to the formation of ion-association complex of 8-hydroxy-2'-deoxyguanosine (8-OHdG) anion with Pyronine Y cation in the pH9.0 buffer. Under the optimal experimental conditions, a new RLS determination for 8-OHdG was developed based on the enhancement(ΔI_RLS) of the system's RLS intensity at 339nm, which is linear to the concentration of 8-OHdG in the range of 50.0²26.4ng/ml. The detection limit is 15.2ng/ml. The proposed method succeeded in the determination of spiked samples with simple and sensitive characteristic.In the chapter 3, a new detection method for 8- hydroxy-2'-deoxyguanosine has been developed with technology of fluorescence resonance energy transfer based on the fluorescence quenching effect. The fluorescent quenching intensity of protamine sulfate(Ps) at 301nm is linear to the concentration of 8-OHdG in the range of 0.50⁷.10μg/ml, at 25℃, in the pH8.2 Tris-HCl buffer solution with the detection limit of 0.18μg/ml. The relative standard deviation was 6.2% and average recovery was 104%(n=6). The proposed method is simple and sensitive, succeeded in the...