Expression And Significance Of Angiogenic Growth And Drug Resistance Related Gene In Gastric Carcinoma

Objectives: Gastric carcinoma is one of the common digestive carcinomas. Studies showed that it was the leading cause of cancer death.As other tumors,the mechanism of gastric cancer is not clear, and the therapeutic effect is not so well.To study the expression of Vascular endothelial growth factor(VEGF), Basic Fibroblast Growth Factor(bFGF) , P-glycoprotein(P-gp) , Multidrug resistance -associated protein 1 (MRP-1) and their relationships with cell apoptosis, and investigate their possible roles in the gastric carcinogenic mechanism,so as to provide evidence for prevention and therapy of gastric carcinoma.Methods: 1 All of the samples were obtained from the 1st and 2nd affiliated hospital of Nanhua University, 30 cases with chronic superficial gastritis(CSG), 30 cases with intestinal metaplasia(IM), 45 cases with dysplasia (Dys) and 65 cases with gastric carcinoma(GC).2 Expressions of VEGF , bFGF , P-gp , MRP-1 were dectected by immunohistochemistry (S-P method) , expression of MRP-1mRNA by Reverse transcriptase polymerase chain reaction(RT-PCR) technique and cell apoptosis by TUNEL method.3 The data were analysized by software SPSS 11.5,and t-test, ANOVA, Chi-square test and Pearson correlation analysis were used to compare the difference between groups.A difference was considered significant if the P value was below 0.05.Results: 1 Expression of VEGF, bFGF, P-gp, MRP-1 showed a gradually increasing tendency from CSG, IM, Dys to GC. Positive rates of VEGF were 23.3% in CSG, 40.0% in IM, 57.8% in Dys and 73.8% in GC respectively; bFGF were 3.3%, 13.3%, 37.8%, 50.8% respectively; P-gp were 6.7%, 16.7%, 33.3%, 52.3% respectively;MRP-1 were 6.7%, 20.0%, 42.2%, 66.2% respectively.There was a significant difference between GC and IM in expression of VEGF(P<0.05), but no significant difference between GC and Dys, Dys and IM(P>0.05); significant...