Enhanced P53 Gene Transfer To Human Nasopharyngeal Carcinoma Cancer Cell Using The Nonviral Vector, HPhA

Gene therapy has become an important strategy in the treatment of various diseases such as cancer, AIDS, cystic fibrosis, tissue engineering and vaccination. In the last ten years, significant advances have been made in gene therapy. In practice, considerable obstacles have emerged. The search for an optimized DNA carrier system for efficient gene delivery is one of the most important tasks in current non-viral gene therapy research. Generally, there are two main types of gene delivery vector: viral and non-viral. Viral vectors, the current favorites for intracellular DNA delivery, suffer from immunogenicity, nonspecificity, and inherent risks of complications such as interference with the activity of tissue-specific promoters. Non-viral delivery systems are expected to have little or no harmful side-effects, an as such, the development of such systems with high efficiency, avoiding toxic and immunogenic problems, is essential and urgent.HPhA, a recombinant histone-like protein from Pyrococcus horikoshii OT3 strain, has compacting activity with DNA as previously reported. Studies show that archaeal histones have primary sequences the histone fold, teritariary structures, and dimer formation in common with the eukaryal nucleosome core...