Inductive Effects Of Rhizoma Drynariac On The Human Dental Pulp Cells In Vitro

Objective: Pulp tissue is the especial connective tissue which is arounded by the sclerous tissues. It is composed by cells and intercellular substances. Pulp tissue has many functions such as nourishment metabolism reparation, etc. Dentinal damage caused by surrounding stimulating can induce the precursor cells in dental pulp cells to be activated, differentiate to odontoblast-like cells, and to format the reparative dentine, then mineralize. At present, it is a main subject that how to promote the pulp cells' activity and potentiality of differentiation. The system of primary cultured human dental pulp cells is the main model to study the pulp cells.With the development of tissue engineering and tissue culture technology in vitro, many scholars cultured the pulp cells of human and many other animals, and studied the cells' biological characters at the cell molecular level. In this study we primary cultured the human dental pulp cells according to the method of tissue-explant, and established the good model to study the functions of human dental pulp cells. We obtained multiplicity cells which not only maintained the source tissue characters but also avoided many factors' interference in vitro.