Effects Of Transforming Growth Factor-β1, Dentin Non-collagen Proteins And Ceramic Dentin Powder On The Human Dental Pulp Fibroblast And Pulp Repair

The production of reparative dentia under pathological inflammatory conditions often results in poor quality reparative dentin containing irregular deposition of collagen matrix, fewer and wid r dentinal tubules, and hypo mineralization. Recently, multiple potent physiological molecules (e.g. growth factors, cytokines, etc.) regulating growth and development, as well as inducing wound healing and tissue regeueration, have been characterized in animals and human subjects. These factors-through mechanisms of receptor binding, signal transduction, and gene activation or suppression-induce proliferation of replacement cells and secretion of extracellular matrix that matures in o reparative dentin. Recent studies suggest that transorming growth factor-β1 (TGF-β1) and non-collagen proteins can (dNCPs) act important role in pulp restore procedure.The TCP-β1 is a large group of growth factors that have important roles in regulating cell growth, differentiation and function. Members of this superfamily have been implicated in aspect", of tooth development and repair of dental tissues after injury, TGF-β1 also Lipregulate the synthesis of extracellular matrices and such stimulation by the isofonn occurred in cultured embryonic mouse dental papillae.Non-collagen proteins, which belong to Dentine extracecllular matrix componeots,have been implicated in differentiation of mesenchymal cells during experimental induction of mineralised tissue formation. Differentiation of odontoblast-like cells from undiffercntiated mesenchymal cells of dental papillae cultured in vitro with isolated noncollagenous extracellular matrix proteins of dentine. But there have been liille report that the induction and differentiation of the two factors to human dental pulp (HDP) cells.In order to investigate the biological effects of the combined use of the two gross tli factors on human dental pulp colls and the possibility of their applicaikm in endodontic therapy, we investigated the effects of two facto s' on proliferation, mineralization matrix secretion and differentiation of IIDP cells in vitro. By direct pulp capping on animals, we observed the effects of the two growth factors though jointly and separately, on the early healing of HDP injury and the formation of deatinal bridge. It was hoped that the study could provide experimental data for better way to preserve vital denial p Hp and improve effectiveness of clinical cndodontic therapy. The present, study include the followings:1. The effects of GNCPs and TGF-β1 on proliferation, ALP activity and mineralization of HDP cells in vitroUsing the enzyme-digest culture technique cultured HDP ceils. Immunohistochemical staining of keratins and waveform fibroins proved that the fibroblasts originated in he mesoderm. In this study, by using MTT method, we observed the effect of dNCPs and TGF-β1 which used jointly or separately on HDP cell proliferation. ALPase activity was analyzed when cells approach to confluent. Von-kossa staining compares the formation of Mineralized nodule. These indicate dNCPs and TGF-β1 increased significantly the cell proliferation and ALPase activity. The two growth factors didn't have overlapping or offsetting effect on the 11DP cell. 2.DNCPs,TGF-β1 modubde colingon compement syntheses in human paipfib rob lastFive-generation human pulps were cultured for 3, 6d supplement with and dent in powder. The expression of collagen were analysis with immunohistochemistry in the different time. The positive signal of collagen I , III were detected in the normal pulp cell. The dNCPs, TGF-1 could stimulate expression of collagen I and downregulate the collagenIII syntheses. These changing of collagen component prove the dNCPs, TGF 1 could induce palp cell into odontoblast.3. induction of dNCPs, TCF-1 on the pulp tissue of organ cultureHuman pulp were cultured for 3, 7,14, 28, 21 days on bone matrix gelatin in DMEM supplementel with different growth factor. The characteristics of matrix were studied through toluidine blue and Mallory s...