Study Of Adult Human Bone Marrow Mesenchymal Stem Cells Differentiation Into Neural Like Cells In Vitro

Objective:Cell transplantation has raised much interest as potential treatment for patients with neurological disease. bone marrow mesenchymal stem cells(MSCs) have recently been shown to have a vast potential for differentiating into many types of mesenchymal cells and even non-mesenchymal cells, such as neural cells under appropriate experimental conditions. This neural phenotypic plasticity allow us to consider the utilization of MSCs as cell material in central nerve system regenerative medicine. Moreover, its separation, cultivation, proliferation, introduction and expression of exogenous gene are easier than neural stem cells (NSCs). So MSCs have be expected to be the most suitable new target cells of cell and gene therapy to cure the neuropathic disease and central nerve system injury. But at present, the mechanism of MSCs differentiated to neural like cells is not clear.so there is not stable and definite method by which MSCs can be induced to differentiate toward neurocytes. In addition, the survival time and generation of differentiated cell are not enough for transplantation, and the function of neural like cells is deficient comparing with true nerve cells. Therefore,our studies focus on the differentiation mechanism of MSCs and how to improve the differentiated ratio and survival of neuron-like cells differentiated from MSCs in vitro.Methods and Results:: Human MSCs were isolated primarily from bone marrow of adult volunteers'iliac and purified sterilely with gradient centrifugation by using Ficoll(1.073×103g/L).The low-density cells including MSCs were cultivated in DMEM containing 20% fetal bovine serum and were purified by passage control. We observed the growth status of MSCs,and identity the immunological characteristic phenotype of CD45,CD44 and CD105 by using FACS , The 6th passage of MSCs were planted into plastic culture flask and induced with BME,DMSO and BHA. The control group did not receive any induced treatment. We observed the morphology change of MSCs, after the induction 6 hours, neuron-specific...