The Preparation Of Platycodin And Its Function To Lowering Hyperlepoproteinemia

Radix platycodonis (Rp) is not only Traditional Chinese Medicine, but also agood source of food with high nutrition and special flavor. Because most of the Rpused to study were uncultivated, the wild breed was chose to be experimental object.In the later years, people know officinal and edible value of Rp more and more, andpay attention to the cultivation and production. In some areas of Shandong Province,such as Zibo city, Tai'an city and Weifang city, Rp are planted in large area, andexported to Japan, South Korea and other countries. So it is necessary to study itsactive component and its biological action profoundly and systematically. With thepurpose of making better use of Radix platycodonis (Rp) and exploiting new medicine,the preparation of the active component of Platycodin and its function to loweringhyperlepoproteinemia were studied in this research.Rp (produced in Chishang town, Zibo city) were chose as object, and themethods of preparing TS(total-saponins ) and its function of lowering blood fat werestudied. Studied documents about preparation of Platycodin, the method of extractionand isolation by supersonic and water (SWO) were set up. SWO method saves lots oftime in contrast to the traditional backfluent-heating (BHF) method, while extractingwith water solvent is safer and saves organic solvent. The rate of production is about6.6%.In contrast, the supercritical CO2 fluid extraction (SCF) was studied, and itshortened the time and caused little pollution. It's also a good method. The rate ofproduction is 2.2%.The extraction was quantitatively analysed. First, it was discriminated bycolor-phenomenon. And then about 23 kinds of monomers were found through MSanalysis. By the means of UV analysis, the rate of production is 86.2% in SWO method,while in SCF method is 92.8%. At the same time, HPLC was used to analyze PD, therate of production is 61.5% in SWO method and in SCF method is 64.0%.In the basis of preparing Platycodin, its function to loweringhyperlepoproteinemia was studied. Male 48 Wistar rats were chose, weight 200±20g.After one week's feed with basic feed, the rats were separated in random into 6 groupsand were named NC, HFC, MC, LTS, WTS, HTS. The rats of NC group were fed withnormal 21 days, the hyperlepoproteinemia model was set up successfully. The nextday, the NC group and HFC group were poured with distilled water into stomachs,while MC group was poured drug (1mg/ml), the latter three groups were poured TS(total-saponins ) solution with the concentration of 5mg/ml, 10mg/ml, 20mg/ml, with2ml injection one time a day. On the 27th day the rats were fed nothing after one night(12h), and cut tails to collect blood. The value of TC, TG, HDL-C, LDL-C, ApoAI,ApoB were determined. And it is found that the LTS, WTS and HTS groups have bettereffect on the function to lowering hyperlepoproteinemia.