The Expression And Significance Of MMP-13,14 And TIMP2 In Human Breast Cancers On Tissue Microarry

Objective: It was said that matrix metalloproteinases (MMPs) can improve tumor invasion and metastasis by degrading extracell matrix.and tissue inhibitor of metalloproteinase(TIMPs) can inhibit tumor invasion and metastasis by degrading the activity of matrix metalloproteinases.By researching the expression of matrix metalloproteinases(MMP-13, MMP-14) and their inhibitor( TIMP-2) in breast cancers and around tumor area., we want to explorâ‘ whether the expression of MMP-13,14 and TIMP-2 is present in breast carcinoma;â‘¡the relation between the their expression and the tumor invasion and metastasis;â‘¢the relation of MMPs/TIMPs and tumor progrssionMethods: 88 cases of breast carcinoma and 46 cases of the tissues nearby were detected on Tissue Microarries. The expression of MMP-13,MMP-14 and their inhibitor TIMP-2 were detected by immunohistochemical P-V9000 method. The results were tested by statistics method square test,Spearman' rank correlation).Results: Among 88 breast cancers, 63 cases were positive for MMP-13 (71.6 %), 86 cases were positive for MMP-14( 97.7%), 65 cases were positive for TIMP-2 (73.9%), In areae of tumor tissue nearby , 24/46 cases were positive for MMP-13 (52.2 %), 38/46 cases were positive for MMP-14( 82.6%), 28/46 cases were positive for TIMP-2 (60.9%), The distinction between the two sites is marked (P<0.05) The expression of MMP-13 and TIMP-2 related with histological grade, and lymph node metastasis (p<0.05), and has no connection with histological grade ,the size of cancer and age(p>0.05). The expression of MMP-14 related with histological grade, lymph node metastasis and age (p<0.05), and has no connection with the size of cancer (p>0.05).But they have no relation each other.Conclusion: (1) The expression of MMP-13 and MMP-14 was stronger in which with breast cancers than breast tissues near to cancer.(2) MMP-13, MMP-14 may be play an important role on the development and lymph node metastasis of breast cancers .It shows that TIMP-2 may be a key action in blocking cancer metastasis.(3) The balance between MMPs and TIMPs is a critical factor which affecte tumor invasion and metastasis.