The Significance And Changes Of Cell Factors In The Process Of Impaired Intestinal Mucosal Barrier By Stress

Objective To found out the mechanism of impaired intestinal mucosal barrier by studying the influence of stress on the barrier, the changes of cells and cell factors and the relationship between them. The identification of protective factors will shed light on the new therapy methods and improved prognosis.Methods 60 healthy Wistar rats were randomized equally to study group, control group and normal group. For the well anesthetized study rats, exposing superior mesenteric artery(SMA), clamping the initiation of SMA and unclamping it one hour later were carried by routine. The control group were the same except for not clamping the SMA. 6 hours later, 1cm intestinal canal 15cm above the ileocecal junction were extracted and studied immunohistochemically, including the expression of TNF-α, VIP, MMP-13 and LN. In addition, the semi-quantitative analysis by VIDAS image analysis system were carried either.Results In the study group, attenuated enteric wall, edema epithelium, short and thin intestinal villi and big space between them were obviously under the microscope. The neutrophil and lymphocyte soaked a lot in the experiment group. Immunohistochemical dyeing showed that the expressionof TNF-a, MMP-13 and VIP in the experiment group were higher and the expression of LN was lower comparing with that in control group(both P<0.05). No difference was found between the control group and the normal group.Conclusion High expression of TNF-a and VIP lead to more expression of MMP-13 under stress. As a result, the intestinal mucosal barrier was destroyed and MODS came into being finally. Therefore, to keep the balance of TNF-a and VIP may be a new method to prevent and cure the impaired intestinal mucosal barrier.