| Objective To investigate the effect of dexmedetomidine and propofol pretreatment on the rats intestinal ischemia reperfusion injury and the impact on the integrity of the intestinal mucosal barrier.Methods The models of II/R were established by clamped the superior mesenteric artery in 40 Wistar rats, which were randomized into four groups. Normal control group(Sham group); intestinal ischemia-reperfusion injury group(II/R group); dexmedetomidine5?g/kg(DEX group) and propofol 10mg/kg(PRO group) pretreatment group. Pretreatment groups, respectively, via femoral vein, which started at 20 min before clamping the superior mesenteric artery(SMA) infusion PRO and DEX. At the end of 2h reperfusion, extracted the blood from inferior vena cava venous, separatded serum for detecting diamine oxidase(DAO)activity. Observed the intestinal epithelial pathological changes through light microscope and made histopathological injury score(Chiu's score).Results Compared to the Sham group, II/R group the activity of DAO obviously increased(P<0.05); Propofol pretreatment could reduced the rise of DAO, Propofol pretreatment could decreased the concentration of DAO(34.29±7.33vs45.46±8.46;P<0.05),but higher than the Sham group and DEX(P<0.05); dexmedetomidine pretreatment significantly decreased the concentration of DAO(23.33±7.06 vs45.46±8.46; P<0.05), its more meaningful than PRO, but higher than the Sham group(P<0.05). Below light microscope, The intestinal tissue of the villi were ulcerated, the intestinal mucosa thinned and the integrity was damaged severely in the II/R group. The protection of DEX to II/R is more effective than PRO, parameters of the two groups still had significant differences(P<0.05);dexmedetomidine pretreatment compared with II/R group, it could decreased intestinal mucosa damaged and it is better to maitain the integrity of intestinal mucosa than PRO.Conclusions After intestinal ischemia-reperfusion injury, the intestinal mucosal barrier integrity was damaged severely. Dexmedetomidine pretreatment could more effective than propofol to maintain the integrity of the intestinal mucosal barrier, reduce the expression of serum DAO, and which is more prominent than PRO in protective role against damaged intestinal mucosa of II/R in rats. |
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