Detection And Signification Of Relationship Between Angiotensin Ⅱ Type1 Receptor And Plasminogen Activator Inhibitor-1 Genetic Polymorphism In Chronic Pulmonary Heart Disease

Objactive Angiotensin Ⅱtype 1 receptor is a important componment of renin angiotension system. Plasminogen activator inhibitor-1(PAI-1) , a kind of glycoprotein, is a member of the SERPIN family.The important step in the progress of fibrinolyic is that tissue plasminogen activator(PA) transforms plasminogen into plasmin, the latter makes fibrin degradation. The main function of PAI-1 is as the fast-acting inhibitor of t-PA.This action prevents the development of fibrinolyic and makes thromsis. The clinical studies show that most patientsof chronic pulmonary heart disease(CPHD) have the syndrom of high risk blood clot and angiotonia. In this study , we investigate the association between the PAI-1 and AT1R gene polymorphisms with pulmonary heart disease on molecular level.Methods The PAI-1 and AT1R genotype in the control group (40 subjects) and the study group(60 patients) is detected by the polymerase chain reaction (PCR).Results ① In the control group, the distribution of the 4G/4G, 4G/5G and 5G/5G genotypes of the PAI-1 gene was 12.5%, 72.5% and 15%;In the study group, the distribution of the 4G/4G, 4G/5G and 5G/5G genotypes of the PAI-1 gene was 15%, 56.7% and 28.3%;There was not significant difference between two groups, 4G allele frequence and 4G/4G genotype /non-4G/4G genotype in control group did not significantly differ from the study group. ② In the control group, the distribution of the AA, AC genotypes of the AT1R gene was 85.0%, 15.0%. In the study group, the distribution of the AA, AC genotypes is 70.3%, 29.7%;There was also no difference in AT1R genotype and the allele frequency between two groups (P>0.05). ③ In the female control group, the distribution of the AA, AC and CC genotypes of the AT1R gene was 87.5%, 12.5% and 0;In the male subgroup, the distribution of the AA, AC and CC genotypes of the AT1R gene was 83.3%, 16.7%, and 0;There was no significant difference between two subgroups, A/C allele frequency and distribution of genotypes in control group did not significantly differ from the study group. ④ In the female subgroup of CPHD, the distribution of the AA, AC and CC genotypes of the AT1R gene was 70.4%, 29.6% and CC 0, In the male subgroup, the distribution of the AA, AC and CC genotypes of the AT1R gene was 69.7%, 30.3% and 0;These percentages in female subgroup significantly differed from the malesubgroup, A/C allele frequency in female subgroup did not significantly differ from the male subgroup. (5) In the female subgroup of CPHD, the distribution of the 4G/4G, 4G/5G and 5G/5G genotypes of the PAI-1 gene was 25.9%, 55.6% and 18.5%;In the male subgroup, the distribution of the 4G/4G, 4G/5G and 5G/5G genotypes of the PAI-1 gene was 6.1%, 57.6% and 36.3%;These percentages and 4G/4G genotype /non- 4G/4G genotype in female subgroup did not significantly differ from the male subroup, 4G allele frequency in female subgroup significantly differed from the male subgroup. (6) In 19 subjects of patients of CPHD accompanying with pulmonary embolism, the distribution of the AA, AC and CC genotypes of the AT1R gene was 84.2%, 15.8% and 0. A/C allele frequency was 92.1% and 7.9%. The percentages of AT1R genotypes was not significantly different between control group and subgroup, the A/C alleles frequency was not significant association between the two groups. ? In 19 subjects of patients of CPHD accompanying with pulmonary embolism, the distribution of the 4G/4G, 5G/5G and 4G/5G genotypes of the PAI-1 gene was 10.5%, 21.1% and 68.4%. 4G/5G alleles frequency was 44.7% and 55.3%. (8) The data of mean Hct was 0.521 ± 0.155 , 0.519 ±0.097 IP 0.483 + 0.056 in the CPHD group, 4G/4G genotype subgroup and the control group. There was not significantly different in control group comparing with CPHD group, 4G/4G genotype subgroup.Conclusion ? PAI-1 4G/5G genotype polymorphism has not a association with CPHD, 4G/4G genotype is not the risk factor of CPHD. ? 4G allely might be a risk factor in female patient of CPHD. (3) There is no association between 4G/4G , 4G allele genotype and CPDH acompanying with pulmonary embolism. ? There was no association between AT1R gene polymorphism and CPDH, with pulmonary embolism. (5) AA and 4G/4G genotype is not associated with the mean Hct of control group.