Proteomic Analysis Of The Plasma Of The Mouse With Liver Regeneration

Background: Liver can complete restoration of the architecture and re-establishment of the specific functions after various types of liver injury. The process of initiation and the control of the final size of the regenerated liver has been the subject of research for many years. However, we still known little about the mechanism of this regenerative process. A better understanding of the process may allow a better selection for patients of partial hepatectomy. Our limited understanding and the complexity of the mechanisms involved have prevented the different factors at differing stages of regeneration. Materials and Methods: Partial hepatectomy(PH) was performed in the operation group mice, and the Sham group was not removed the liver lobes. The plasma of the both group run two-dimensional electrophoresis gels, separately. After silver staining, the differential expression proteins were analyzed by Imaging Master 2D analysis software. We used proteomics technology to globally examine the plasma from mouse operated with partial hepatectomy. This research has been preformed in four time points (after surgery 12h, 24h, 48h and 72h). Through comparing with the Sham and operation groups, we found some changed proteins, comparatively analysis these proteins in the four operation groups sequentially. By observed the new appearanced, lost and changed in density protein, we could search the key protein in the progress of liver regeneration.Results: Analyzed the 24 gels we gotten totally by 2-D ImageMaster. There was 2 points in the 12h and 24h PH teams, none in the Sham teams. 1 point express highly in the 12h, 24h and 48h PH teams than the Sham teams, but at time of 72h there was no difference. 2 points express highly in the 12h and 24h PHteams than the Sham teams, but no change in the 48h and 72h group. 3 spots of the 5 protein spots significantly changed were determined by MALDI-TOF MS, these greatly altered proteins were identified to be fumarylacetoacetate hydrolase and transferrin. By reviewed data from the published papers, we found these two proteins have correlations with liver regeneration, and they could be useful to the liver regeneration mechanism research.