The Relationship Between PPARγ And CAPN10 Gene Polymorphisms And Type 2 Diabetes Mellitus In Hubei Han Chinese

Type 2 diabetes mellitus (T2DM) is complex disease characterized by elevated blood glucose level that results from insulin resistance and deficiency in 8 cell secretion. There are 30 million diabetic patients in our country, accounting for the one fifth of diabetic patients in the world. The proportion of T2DM is around 95%. The genetic factor plays the key role in the pathogenesis of T2DM. Identification of susceptibility genes of T2DM would have important implication for primary prevention of T2DM and screening of high-risk individuals.The PPARy gene and CAPN10 gene are two important candidate genes of T2DM. To investigate if the polymorphisms of these two genes are associated with T2DM in Han Chinese from Hubei, a total of 52 sibpairs and 124 case-control were recruited to study the association between PPARy2 Prol2Ala and C1431T polymorphisms, CAPN10 SNP19 polymorphism and T2DM. The results were as follows:1. There were not significant differences in genotypes and allele frequency distribution of Prol2Ala polymorphism between subjects with T2DM and controls. ANOVA showed that Prol2Ala polymorphism was not related to the clinical phenotype in both diabetes and controls. In the samples of sibpairs and all subjects studied Logistic regression analysis showed the Prol2Ala polymorphism was not related toT2DM. In the samples consisting of T2DM and unrelated controls the polymorphism was not associated with T2DM, However, after adjustment for age,sex,hypertension and obesity the polymorphism was positively and significantly related to T2DM. The study also showed the hypertension was the independent risk factor of T2DM in sibpairs of T2DM patients and controls and all subjects studied and age was the independent risk factor of T2DM in samples of T2DM patients and unrelated controls and all subjects studied. Multivariate Logistic regression analysis indicated that age, hypertension, WHR (not the Prol2Ala polymorphism) were independent risk factor of T2DM.2. There were not significant differences in genotypes and allele distribution of C1431T polymorphism between subjects with T2DM and controls. Logistic regression analysis showed the C1431T polymorphism was not related toT2DM in the samples of sibpairs of T2DM patients and controls and all subjects studied. In the sample consisting...