Levels Of IL-18 In Experimental Hepatic Injury In Rats And Its Relationship With The Protective Effect Of Lipo-PGE1

BackgroundHepatitis is one of the common diseases to endanger people of health. The treatment of the serious hepatitis has been a very thorny clinical question all the time. The mechanism of the disease is complicated, refers to the duplicate and attack of hepatitis virus, the maladjustment and failing of immune function, the action of various kinds of cell factors, and microcirculation obstacle.Interleukin-18 (IL-18) is a kind of important cell factor found in recent years, it is mainly produced by activated macrophage cell and hepatic Kupffer cell, and its levels can reflect severity of hepatic injury. The level of IL-18 contributes to judging the prognosis of the hepatic diseases. Lipo-PGEl is a little spheroid preparation to enclose prostaglandin E1 (PGE1) in the little ball of fat, utilize its special affinity to give play to the target to enable PGE1 to assemble in the pathological change position function, and make it avoid deactivation in the lung, thus reduce the consumption of prostaglandin E1 greatly, obviously reduce its stimulus and inflammation to the blood vessel, lengthen function time.ObjectiveTo study the level of IL-18 in experimental hepatic injury in rats and its relationship with the protective effect of Lipo-PGE1.Methods32 SD rats were divided into treatment group and model group randomly. D-galactosamine (D-Gal, 1.4g/kg) was injected into the abdominal cavity of all rats, to set up the animal model of experimental hepatic injury. Sterilization physiological saline was injected into abdominal cavity of model group (16 rats). Lipo-PGE1 (30 ug/kg) was injected into abdominal cavity of treatment group (16 rats). At the time points of 0, 6, 12, 24h, the changes of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and liver IL-18 were measured.ResultAfter injection of D-Gal, the levels of serum transaminases (ALT, AST) and liver IL-18 were increased significantly (P<0.05). The histopathology showed infiltration of inflammatory cells, dilatation of liver sinusoids and proliferation of Kupffer cells, with subsequent hepatocytes apoptosis and necrosis. After the treatment of Lipo-PGEl, the changes of serum transaminases, liver IL-18, and liver histopathology were improved simultaneously.Conclusion? EL-18 is involved in the hepatic injury.? Lipo-PGEl can protect the liver from injuries.?The protective mechanism of Lipo-PGEl may be done by decreasing the levels of IL-18.