| Objective: To study the effect of Lipo-PGE1 on intestinal injury due to blood reperfusion after venous congestion by portal occlusion in rats.Methods: Thirty SD rats were randomly divided into 3 groups(n=10), the congestion reperfusion group(CR), the Lipo-PGE1 group (LP) and the control group(CO). In group CR and LP, the portal flow was resumed after the portal vein had been occluded for 45min, while in group CO the portal flow was not interrupted. Additionally, 0.2μg Lipo-PGE1 was injected via jugular vein before, during and after the portal occlusion in group LP , respectively. After he intestine having been reperfused for 60 min in group CR and group LP, jejunum samples in 5cm length were collected for MDA and MPO determination as well as pathologyical biopsy. Then the intestine lumen was washed with 10ml normal saline and 2 ml of glucose(5%) was injected into the lumen. Finally the glucose concentration in portal blood was determined at intervals of 15min in each group.Results: In group CR, pathologyical biopsy showed that the intestinal mucosa was seriously damaged and the mucosal lesion was much worse than that of group CO in Chiu's Classification(P<0.05). Glucose concentration in portal blood at time point of 0 min, 15 min, 30 min, 45 min, and 60 min was respectively 2.550±0.303 mM, 4.560±1.280 mM, 5.820±1.501 mM, 6.420±1.293 mM and 4.770±0.685 mM, and with time of peak at 45min. In contrast, glucose concentration in group CR at each time point, except for 0 min, was markedly less than that of group CO(P<0.05). In group CR, MDA concentration and MPO activity were 1.853±0.556 nmol/mgprot and 1.188±0.346 U/g, respectively. They were all remarkably higher than those of group CO(P<0.05). Histological examination, however, presented that the mucosal injury was markedly attenuated in group LP, and the injury degree was much lower than that in group CR with Chiu's Classification(P<0.05). Glucose concentration of group LP at different time point was 2.740±0.552mM, 6.240±2.765mM, 8.040±2.817 mM, 7.570±1.852 mM and 5.830±1.248 mM, respectively. The peak of glucose concentration in group LP appeared at 30min ahead of that of group CR. Glucose concentration at 30min, 45min of group LP was remarkably higher than that in group CR(P<0.05); but there were no significant changes at 0min, 15min and 60min between the two groups(p>0.05). Intestinal MDA concentration and MPO activity in group LP were 1.232±0.309 nmol/mgprot and 0.854±0.143 U/g, respectively. Each of them was markedly lower than those of group CR(P<0.05). In group CO, glucose concentration at different time point was respectively 3.150±0.978 mM, 8.170±1.592 mM, 9.970±2.008 mM, 8.240±2.036mM and 6.330±1.296mM. The peak of glucose curve appeared at 30min. Pathological examination indicated that the intestinal mucosa wasn't injured generally. Intestinal MDA concentration and MPO activity in group CO were 0.985±0.454 nmol/mgprot and 0.575±0.185 U/g. Conclusion: (1) Blood reperfusion after venous congestion could induce the intestinal damage and depress the ability of intestine to absorb glucose in rats. (2) When the rats treated with Lipo-PGE1 the intestinal injury was remarkably attenuated and the ability to absorb glucose was greatly enhanced. (3)Hyperoxidation of lipid in intestine happened during the congestion and reperfusion. But there was no reason by which Lipo-PGE1 could alleviate the hyperoxidation of lipid in intestine. (4) A phenomenon was noted that there were a great many of polymorphonuclear leukocytes in the injured intestine and the mechanism of the phenomenon is worthy to investigate. |
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