Effects Of Pulse-activating Powder Combined With The Roast Glycyrrhiza Decoction Adjusted Juice Ⅱ On Cultured Myocardial Cells Infected With Coxsackie Virus B₃

In this study, we investigated anti-viral effects and the protecting mechanisms of pulse-activating powder combined with the roast Glycyrrhiza decoction adjusted juice II for the cultured rat myocardial cells infected with Coxsackie virus B₃ (CVB₃).Myocardial cells, which were obtained from the ventricular myocardium of newborn Sparaque-Dawley rats to make the beating myocardial cells, were incubated for 48 hours and inoculated with 10³CID50 of B₃ virus as experimental model of VMC. All drug directly were administrated to the culture medium of myocardial cells in vitro. All models were randomly divided into fore group: normal group, control group, control of positive treated group: pulse-activating powder combined with roast Glycyrrhiza decoction adjusted juice (excluding Polygonum cuspidatum water exact) treated group(PGP â… ), and pulse-activating powder combined with roast Glycyrrhiza decoction adjusted juice (including Polygonum cuspidatum water exact) treated group(PGP II). Pathogenic changes and cells beating of normal myocardial cells and myocardial cells infected with CVB₃ were observed by inverted microscope at 24, 32, 48, 60 and 72 hours after viral inoculation, the ultrastructure of myocardial cells at 72 hours after virus inoculation was observed by electronic microscope, the activity of Lactate Dehydrogenase (LDH), Creatine Phosphate Kinase (CPK) were determined by biochemical semi-automatic analyzing instruments The results showed that the PGP â…¡ significantly alleviated the pathogenic changes of myocardial cells infected with CVB₃, improved the cell beating and percent of cell beating (compared with viral control group, P < 0.05), alleviated the ultrastructive changes of myocardial cells; inhibited obviously from releasing enzyme of myocardial cells infected with CVB₃ (compared with viral control group , P < 0.01 ).Effects of the PGP â…¡ were better than that of the PGP â…  (compared with control group P <0.01). In this experiment, it is found that the PGP II have anti-viral effects for myocardial cells infected with CVE^and significantly protective effects for the changed myocardial cells infected with CVB3, and the stabilizing cell membrane and increasing the energy to cells probably is one of protective mechanism. Effects of the PGP II are better than that of PGP I, and therefore we suggest to treat the VMC with PGP II adding the Polygonum cuspidatum in clinic.