An Experimental Study Of Effect Of Nm23-H₁ Gene On The Chemotherapeutic Sensitivity Of Bladder Cancer In Vitro

Object: by means of transfecting the nm23-H1 gene into human bladder cancer cell lines T-24 cell, the cell lines T-24 cell of steable expression was set up. To evaluate the effect of exogenous human tumor suppressor gene nm23-H1 on the chemotherapeutic sensitivity of the bladder cancer cell lines T-24 cell in vitro. Methods: The higher pure pCMV-Neo-Bam of nm23-H1 was made out by the way of gene transfection, and the pCMV-Neo-Bam of nm23-H1 was identified, the plasmid of nm23-H1 was transfected into human bladder cancer cell lines T-24 cell by the method of Lipofect AMINE, and the expression of nm23-H1 were assayed by means of immunohistochemical technique. MTT assay was used to assess the cell proliferation effect of the chemotherapeutic medicines on the bladder cancer cell lines T-24 cell transfected with nm23-H1, and the cell apoptosis was measured by flow cytometry. Result: in vitro, the grows of T-24 cell With cisplatin (CDDP) , transfected with nm23-H1, obviously was subjected to be repressed ; and the cell apoptosis was to run away to add . Conclusion: The cell lines T-24 cell of pCMV-Neo-Bam- nm23-H1 was successfully set up. Gene of nm23-H1 can enhance the chemotherapeutic sensitivity with cisplatin (CDDP), it's mechanism may be connected with Na~+/K~+-ATPase. This will supply the reference of the gene-chemical therapy for the bladder cancer.