The Laboratory Study About The Proliferative Effect Of Aspirin And Celecoxib On Breast Cancer Cells MCF-7

Breast cancer is a frequent tumor among women,incidence rate and mortality rate of that have beenincreasing for last twenty years. Breast cancer has becomethe most frequent cause of death among women. Recently, alarge of studies discovered that COX-2 over-expressed incolon carcinoma, gastric carcinoma, esophageal carcinoma,pancreatic carcinoma, lung carcinoma, breast cancer and soon commonly, that suggested COX-2 probably participated indevelopment of many tumors. NSAIDs is a antipyreticanalgesic, because it has good therapeutic effect thatrelieving fever, relieving pain, anti-inflammatory, andrelieving rheumatism, it has been one of the most commonlyused drugs. Nearly, we discovered that NSAIDs has theantineoplastic effect besides relieving fever andparegoric efficacy unexpectedly. Above all, aspirin caninhibit COX-1 and COX-2 simultaneously, and decreaseincidence rate of breast cancer. But the side effect ofnon-selective COX-2 inhibitor if long-term administration,especially the effect on digestive tract that confines itsapplication in preventing breast cancer. A selective COX-2inhibitor, celecoxib can prevent and treat colon cancer,that has been confirmed, FDA gave approval that celecoxibcould be used to treat familial polyposis on 2000, and thatcelecoxib was used to treat colon tumor had enteredclinical trial. At present, findings manifest selectiveCOX-2 inhibitors have preventive and therapeutical effecton breast cancer, furthermore they can cut down distantplace metastasis. Now, COX-2 inhibitors are used toclinical practice gradually, and they usually combine withother medicines. Initial researches indicated thatselective COX-2 inhibitors had preventive and therapeuticeffect on mammary cancer, they tolerance was better. Inthis study, the inhibitory effect that NSAIDs and arimedexon breast cancer cell MCF-7 was detected by MTT assay. Fromthe results, we comprehend that the effects of NSAIDs andarimedex on the growth of MCF-7.In this study, we adopted MTT assay to detect theseeffects of 2.5mM, 5.0mM, 10.0mM aspirin and 30uM, 60uM,120uM celecoxib on the growth of human breast cancer cellMCF-7.The results showed NSAIDs can inhibit the growth ofMCF-7, and these inhibitory effects were time-dependent anddose-dependent. The most inhibition ratio of aspirin andcelecoxib were 68.88% and 87% respectively, the inhibitionratio of 2.0uM ADM on MCF-7 for 72 hour was 86.3%. Theseresults that anastrozole combining aspirin and celecoxibrespectively affected on MCF-7 showed NSAIDs combiningwith anastrozole had synergism antineoplastic effect, andthese effects were dose-dependent.From these results, we concluded that both aspirin andcelecoxib had inhibition effects on the growth of humanbreast cancer cell MCF-7,and they were time-dependent anddose-dependent inhibitors. The two inhibitors can achieveidentical inhibition effect on MCF-7 compared to ADM.Aspirin and celecoxib combined with anastrozole havesynergism antineoplastic effect, which weredose-dependent. This study contributes to the method thataspirin and celecoxib could be used to prevent and treatbreast cancer.