VB₆ Improves Learning-memory Ability, Motor Dysfunction And Synaptic Plasticity Of Hippocampus In Cerebral Palsy Rats

Objective: The effects of long-term intake of VB₆ on the ability of learning-memory, motor dysfunction and synaptophysin immunoreactivity in CA3 area of hippocampus in cerebral palsy rats. The possible mechanism of improving learning-memory ability, motor dysfunction in rats treated with VB₆ was revealed further.Methods: Aged 14 days Wistar 72 rats were randomly divided into 4 groups, contral group(CC, n=18), sham surgery group (CS, n=18), cerebral palsy group (CM, n=18) and. cerebral palsy treated with VB₆ group (CM+VB6, n=18). An improved Philip method was used to model cerebral palsy. Morris water maze test (MWMT) was used to detect the changes of learning-memory ability, including escape latency (EL) and swimming distance (SD) for searching submerged platform;Beam walking test (BWT) measuring the latency of different beam was used to detect the changes of coordination and balance function;Footprint pattern test (FPT) was used to detect the changes of gait, including left/right stride length (LSL,RSL), front-base width (FBW), hind-base width (HBW), distance from left/right front footprint/hind footprint overlap (DLFHFO,DRFHFO);The synaptophysin (SYN) in CA3 area of hippocampus was revealed with immunohistochemical method.Results: (DMWMT: EL and SD of CM+VB6 rats were shorter than that of CM group from the 12th day to the 20th day(P <0.05). EL was undifferentiated from the 19th day to the 20th day, and SD was undifferentiated on the 20th day compared with between CM+VB6 and CC rats(P >0.05). EL and SD were undifferentiated compared with between CS and CC rats (P >0.05). ?BWT: Latency of different beam of CM+VB6 rats were shorter than that of CM group from the 3rd day to the 9th day(P <0.05). Latencys of beam 100 cm><784 mm2 ,100 cmxl44 mm2 were undifferentiated, but latency of beam 100 cm><25 mm2 was longer on the 9th day compared with between CM+VB6 and CC rats(P>0.05), which demonstrate the coordination and balance function can't be recoveryed literally with increasing difficult of tasks. (3)FPT: The longer RSL from the 5th day to the 9th day, and the shorter HBW and DRFHFO from the 7th day to the 9th day compared with between CM+VB6 and CM rats(?<0.05), which demonstrate the improving abnormal gaits. The longer RSL, HBW and DRFHFO from the 1st day to the 9th day, which demonstrate the abnormal gaits can't be recoveryed literally. ?Result of SYN: The positive immunoreaction labeled SYN in CA3 area of hippocampus revealed more increase in CM+VB6 rats than that in CM on the 20th day(P <0.05), and more decrease in CM+VB6 rats than that in CC (PO.05), and was undifferentiated in CS rats to CC on the 5th day, 10th day and 20th day (P>0.05).Conclusions: ?VB6 improves on the motor dysfunction in cerebral palsy rats, improves abnormal gaits, and recoverys coordination and balance dysfunction except that of the most difficult task. ?VB6 improves the ability of learning-memory impaired in cerebral palsy rats. (3)VB6 enhances the synaptic plasticity in CA3 area ofhippocampus by increasing the synaptic quantity.