The Expression Changes Of The Synaptophysin In The Hippocampal Formation During The Spatial Discriminative Learning And Memory In Rats

Purpose With immunohistochemical methods under the light and eletronic microscopic level, together with the computer analytic techniques , the expression changes of the synaptophysin in the hippocampus of the rats that have obtained the abilities of spatial discriminative learning and memory established by water maze during the different periods will be studied, after they stopped training. Probing into the relationship between the amenia of the spatial discriminative learning and memory with the expression changes of the synaptophys in. This study will provide the morphological baseline for the further studies on the synaptic plasticity of the spatial discriminative learning and memory. Methods SD male rats were randomly divided into three groups: that were the model group, the swimming group and the control group. Every group was further randomly divided into four subgroups according to the days they stop training. The model means the rats that were trained for 7 days by water maze. After these rats reached the model standards of spatial discriminative learning and memory, they were trained 14 days continuously, that is, they had been trained for 21 days. Then, the rats were sacrificed and the hippocampus was drawn at the different time point when they stopped training. With the immunohistochemical methods, the synaptophysin expression was examined. Under the light microscopic level, the time and the distribution of the synaptophysin expression was carelu] lv observed, and their differences were compared. The correctedoptical density of the positive synaptophysin staining in the hippocampus was measured, and the expression?s quantity analyses were made by the computer analytic system, moreover, the positive synaptophysin mmunostaining particles under the subcellular level were also localized with immunoelectronic microscopic methods. All the data were analyzed by the software of SPSS 8. 0 for windows. Results 1 On the training day, the average latent, the average running time and the correct ratio of all three quotas had reached the standard on the reference. That is, the rats had obtained the abilities of spatial learning and memory. 2 Their training performance in water mazes on the day when the model rats were sacrificed also accorded with the standards of the model. 3 A significant increase (P0.05). 5 No quantity and distribution differences between the swimming group and the control group (P>O.05). 6 The positive synaptophysin mmunostaining particles were localized in the perakayal of neuron and the nervous terminals . In the nervous terminals, they not only exist on the surface of the SSVs, but also on the LDCVs .And the positive terminals make symmetric synaptic contacts with the negative one. Gonc I us ions rhe animal models of spatial discriminative learning and...