The Expression Of Bcl-2 And Surviving On Vascular Endothelial Cells In Different Stages Of Vessel In Hemangiomas

PrefaceHemangiomas is a benign blood vessel pathological change, and it usually occurs in children. The hemangiomas occurred in the face and oral are more than 60% of all of the bodies. Pathophysiology of hemangiomas is still unknown. Apoptosis is responsible for subsidise of hemangiomas cells.Bcl - 2 is one of the inhibiting factors of apoptosis, it is separated from non — Hodgkins lymphoma by Tsujimoto which is a cancer gene. There are many researches of Bcl - 2, however, Bcl - 2 antigen expression is rarely reported in blood vessel endothelial cells in different phases in hemangiomas.Survivin is considered as one of the inhibiting factors of apoptosis at present, it is a new member of the inhibiting albumen family of apoptosis. Although there have been many studies of Survivin in the past years, Survivin expression is rarely reported in Hemangiomas.We examined the expression of Bcl - 2 and Survivin in different phases in hemangiomas and on vascular endothelial cells in vascular malformations wising immunohistochemistry to further elucidate the role played by them in the patho-physidogy of hemangiomas and regression.Materials and MethodsAn SABC immunhistochemical technique was used of to examine the expression of Bcl - 2 and Survivin monoclonal antibodies in 63 infantile hemangiomas and vasallar malfonmations. Fresh specimens were divided to be either forimmunohistochemical study or stained in hematoxylin eosin. The distribution of Bel - 2 and Survivin molecules on endothelial cells was scored semi - quanti-tatinely by two individual observers at x200 magnification on the whole specimens as fallows( - ) when endothelial staining was absent;( + ) when staining on endothelial sections was focally positive;and ( + + ) when the endothelial sections were diffusely stained. Statistical analysis was performed using x test.Results1. Expression of Bel -2;We observed a significant difference (P <0. 001) of Bel - 2 expression on endothelial cells between the proliferation and the degradation phase of hemangiomas. We found high levels of Bel - 2 expression on all the endothelial cells surrounding virtual lumens in most of the proliferating hemangiomas (22/23) and vassular malformations (17/19). However, there was scarcely expression of bcl -2 in the well differentiated vessels. In contrast, Bel - 2 was poorly or no expressed on endothelial cells in most of the degradation hemargiomas (15/21).2. Expression of Survivin: We observed no difference (P > 0. 5) of Survivin expression on endothelial cells between the proliferation and the degradation phase of hemangiomas. We found high levels of Survivin expression on all the endothelial cells surrounding virtual lumens in most of the proliferating hemangiomas (18/23) , vassular malformations (16/19) , and degradation hemangiomas (14/21). In contrast, Survivin was poorly or no expressed on endothelial cells in these phases.DiscussionInfantile hemangiomas is a benign blood vessel pathological change, and it usually occurs in children. The hemangiomas occurred in the face and oral are more than 60% of all of the bodies. Pathophysiology of hemangiomas is still unknown. Apoptosis is responsible for subsidise of hemangiomas cells. Characterizing the life cycle of infantile hemangiomas into two phases of clinical develop-merit. The proliferating phase (13 months - 12years). In the proliferating phase, hemangiomas are charactenized by an important proliferation of their en-do thelial cells organized in " islet" surrounding poor lumen formation. Differentiation of the ressel proliferation with large lumen formations lined by plump on flattened endothelial cells firstly begins at the periphery of the dense cellular areas and progressively involves all the vascular proliferation. In contrast, the degradation phase is characterized by a decreased cellularity, well differentiated capillaries and intenstitial fibrosis.Bel - 2 is one of the inhibiting factors of apoptosis, it is separated from non— Hodgkins lymphoma by Tsujimoto which is a cancer gene. It lies in the 28th chromosome q21, and is composed of 3 parts, coding Bel -2a and Bel -2b albumen. Bel -2 can restrain apoptosis of cells by many factors. Present research shows that Bel - 2, P53 , Caspases, C - myc, Fas, TNF, Cyclin, ras are inhibiting factors of apoptosis all. This study clearly demonstrated that high levels of Bel -2 expression in the proliferating hemangiomas. In contrast to the degradation phase, there was a significant difference (P<0.05).Survivin is considered as one of the inhibiting factors of apoptosis at present , it is a new member of the inhibiting albumen family of apoptosis. There are many evidences to show that Survivin exert restrain apoptosis by control caspase- 3 and caspase - 7. This study clearly demonstrated that low levels of Survivin expression in the proliferating and degradation hemangiomas. there was no significant difference ( P > 0. 05).In conclusion, different expression of antibodies on vascular endothelial cells in the proliferating and the degradation hemangiomas provides circumstantial evidence about the phathophysiology of hemangiogenesis and regression. Our results sustain the hypothesis that Bel - 2 may play a role during the early stage of angrogenesis, and take part in the pathology of hemangiomas. Further research would be necessary to determin the role that Bel - 2 play in degradation methods of hemangiomas. At last, further investigations should be necessary to understand the physic opathogenesis of hemargi genesis and regression in infantile hemangiomas. Understanding the interactions between molecules which at in angiogenesis will help to find new treatments against complicated hemangiomasand tumors.Conclusions1. This study confirmed high levels of Bel - 2 expression on vascular endo-thelial cells in proliferating phase in infantile hemangiomas, and a significant difference in contrast to the degradation hemangiomas. These results indicated that Bel -2 may play a role in the later stage of angiogenesis.2. This study confirmed high levels of Survivin expression on vascular endo-thelial cells in proliferating phase in infantile hemangiomas, and no significant difference in contrast to the degradation hemangiomas. These results indicated that Survivin may not play a role in the apoptosis of infantile hemangiomas.