An Experimental Animal Model For Cryptosporidium Human Strain And Phylogenetic Of Cryptosporidium Based On Different Gene Locus

Cryptosporidiosis is an important apicomplexan disease of considerable medical and veterinary importance. Till now, there is still no effect drug to control the disease. Mitochondrion is an organelle which contain the most protein and enzyme in eukaryotes, so the mitochondrion of Cryptosporidium may be an effect potential target of drugs. Recent studies have provided evidence for a mitochondrial derived compartment in this parasite. But the organelle has some difference to that of other apicomplexan parasites. This organelle appears to lack its genome, and thus must be entirely dependent on nuclear-encoded proteins.1. To estimated the affection of the two common immunosuppression methods on discharging oocysts rule. Methods: 19-days-old mice were divided into two groups randomly, and then different immunosuppression methods were adopted by this experiment.The first group was given the dexamethasone (DEX) by drinking water ,while the second group were given intragastrically with pipe, after 3 days of inoculated with cryptosporidium oocysts(CSO), each mouse was inoculated with 1 ×10~6 CSO. Result: The mice discharged the oocysts in the first day after inoculated, and in the DIP6, the counted number of the CSO increased obviously, 3 peaks of occysts discharging appeared, and then dropped gradually, during this period, some of the mice died, but no diarrhea symptom was discovered .Comparing with the two groups, It was concluded that the number of the oocysts discharged by the second group is larger than that of the first one, and so does the persistence period of the peak. Conclusion : After using both of the two immunosupprssion methods, we can establish the animal model successfully when inoculated by CSO, and the efficacity of the second method is better than the first one.2 In order to further understood the endogenous development of Cryptosporidium human strain in mice. The author used 19-day-old mice of Kunming strain as model animals, random divided into 8 eight groups, 3 dosage of Cryptosproridium inoculated, than determined the parasitic peculiarity of the parasite with the change of time and inoculated dosage. At the time 4h, 8h, 12h, 48h, 72h post infection killed one mouse of...