Protection Of Pinacidil On Intestinal Epithelial Cells' Mitochondrion In Burnt Rats

Object: The intestine is one of the most sensitive organs on ischemia and anoxia. Since intestinal mucous membrane barrier was damaged after burn, microorganism and endotoxin entered into blood from intestinal tract, which easily resulted in systemic inflammatory response syndrome (SIRS) and even more, multiple organ dysfunction syndrome (MODS).Mitochondrion, an organelle for translating energy, is very sensitive to ischemia and anoxia, which can cause disturbance of mitochondrial structure and function.Recent studies found that ATP sensitive K+ channel (KATP) opener could protect myocardia from ischemia and hypoxia. Its pharmacological role targeted myocardial mitochondrion membrane KATP channel (mitoKArp). However, there wasn't any report about this kind of protection on intestinal mucous membrane damaged by ischemia and anoxia.Therefore, this study aimed at searching for pharmacological protection of pinacidil (Pin), a mitoKATp opener, on intestinal mucous membrane damaged by severe burn.Methods:(1) After establishing a model using intestinal epithelial cells (HIC) damaged by reactive oxygen species, intestinal epithelial cells mitochondrial allomeric function, cytochrome C release, mitochondrial membrane potential and pinacidil protection on intesting epithelial cells mitochondria were observed.(2) After establishing 30% TBSA III0 burn model, ROS, MDA and SOD in plasma and intestinal mucous as well as cytochorme C, mitochondrial respiratory function and pathological morphology changes in rats treated or un-treated with pinacidil were observed.Results:(1) In vitro, It was found that HIC cells' mitochondrial functions descented; cytochrome C release increased, while mitochondrial membrane potential reduced. However, pinacidil could improve mitochondrial function significantly.(2) In burnt rats, ROS and MDA elevated in plasma and intestinal mucosa. SOD and mitochondrial RCR decreased. Mitochondrion swelling occurred in burned rats, too. The remarkable changes appeared at 6 hour-point after burn. Pinacidil could improvemitochondrial functions and decrease pathomorphology damage of intestinal mucosa.Conclusion:(1) Pinacidil could protect intestinal epithelial cells from reactive oxygen species' injury in vitro(2) Pinacidil could significantly protect intestinal mucous membrane at early stage in burnt rats in vivo.