The Relationship Between Diabetic Nephropathy And The Expression Of VEGF And SICAM-1

[Objective]Diabetes nephropathy (diabetic nephropathy,DN) is diabetes one of mostserious three big illness complications,with heart disease, the cerebro vasculardisease with is the diabetes important cause of death 1 diabetes patient 5-10 year 30-40% has DN approximately, 2 diabetes patients 10-20 year 15% has DN approximately. The pathological change implicates the renal glomerulus, the kidney blood vessel and the interrenal nature, finally may send the end last stage kidney to fade(ESRD), seriously affects patient's survival and the quality of life. Since the 90's have discovered the blood vessel bast growth factorgradually(Vascular endothelia growth factor,VEGF), the intercellular space adherency member -1 (SICAM-1) has close relation with diabetes capillaries pathological change occurrence development, but DN patient blood serum VEGF, The SICAM-1 level and its two between relational research are few.This experiment through examination different stage DN patient blood plasma VEGF, the SICAM-1 level, discusses VEGF, SICAM-1 and the DN relevance.[Methods]The research object is 89 examples 2 diabeteses patients and 20 examples healthy collators. Chooses in 2004-2005 year JI NAN hospital to be hospitalized and the out patient service DM patient 89examples, the male 46 examples, the female 43 examples, the age is23-77 years old, the average 54. 5 earth 11.1 years old, tally in 1999 the WHO diabetes to diagnose the standard 2 diabeteses patient 86 examples, 1 diabetes patient 3 examples. Selected condition:Removes the heart, the lung, live disease,theuropoiesis is the infection and other kidney diseases;Removes otherendocrine diseases, the knot contracts the organization to get sick with the tumor;Has not had diabetes alkone sickness acidosis and other acute illness complications in 1 month;Removes other like has a fever,influencealbuminuria the and so onstrenuous exercise factor;In the near future use tokidneyharmfulmedicine;Through inquiry medicalhistorymedicalexaminationelectrocardiogramassistance examinationand so on eyeground examination and myo-electrograph removes other serious illness complications. (UAER) divides into three groups according to the urine albumin excretion rate:1. normal albuminuria group DM.Group UAER<20ug/Min 37 examples, male 21 examples, female 16 examples.2. microalbuminurias groups MA Group UAER 20-200ug/Min 31 examples, male 16 examples, female 15 examples.3 .clinical albuminurias groups ODN GroupUAER>200ug/Min 21 examples, male 11 examples, female 10 examples.The establishment healthy healthy control group 20 examples, the male 11 examples, the female 9 examples, the average age 50.47 earth 8.59 years old, are anexception hypertension, diabetes, the high blood fats, the heart, the lung, the liver, kidney disease and are good, situation and so on malignant tumor, surgery and flesh wound. All is determined the examination the body weight index (BMI), the blood pressure, the empty stomach blood sugar (FBG), meal the latter 2 hours blood sugars (PBG),the saccharification hemoglobin (HbAjC), the kidney merit, the blood fats and so on. Contains filling in the ABC-ELISA law determinationblood serum with the double immune body VEGF, the SIC AM-1 density. [Results]In SPSS11. Osoftwares carries on the data analysis. Carries on the variance analysisseparately to each kind of data, pairs t to examine and to carry on the correlation analysis.l.In the blood serum the VEGF level in healthy control group is 291.181 ± 50.44 pg/ml, in DM, MA, in the ODN three groups respectively is 368.84 ±41.32pg/ml,437.28 ± 58.87pg/Ml,560.23 ± 50.33 pg/ml DM, MA, in the ODN three groups of patients blood serums the VEGF density obviously advances, compares with healthy control group has the extremely remarkable difference ( P<0.01);MA, ODN group DM compares the group the patient obviously advances ( PO.01);The ODN group increases Gauguin the MA group to be more obvious than (PO.01).2. In the blood serum SICAM-1 level normal in healthy control group is325.01±28.96 ng/ml, in DM, MA, in the ODN three groups respectively is 496.77±152.33 ng/ml, 603.07±146.76ng/ml, 612.56±126.38 ng/ml. Discovered patient DM, MA, the ODN three groups of blood serums densities obviously advance, compares with healthy control group has the remarkable difference (P<0.01);The MA group patients advance compare the DM group patients to be obvious, but does not have thesignificance difference (P>0.05);The ODN group compares DM, MA advances,Does not have the significance difference (P>0.05).3.Diabetes and in hypertension patient blood serum VEGF,The SICAM-1 density compares diabetes not to have hypertension group patients to advance, but does not have the significance difference(iJ>0.05).4.In diabetes group patients, in the blood serum VEGF and the sVCAM-1 density presents the correlation (r=0.763,P<0.01);The blood serum VEGF density and UAER elevate presents the correlation(r=0.757,P<0.01);The blood serumSICAM-1 density and UAER elevate also presents thecorrelation (r= 0.765,P<0.01).5.In diabetes patient blood serum the SICAM-1 density and the low density lipoprotein cholesterol (LDL-C) the level presents the correlation (r= 0.301,P<0.05), with HbAlc, the blood pressure, BMI does not have the correlational dependence;In the blood serum the VEGF level and HbAiC become is being connected (r=0.335,P<0.05),With between the blood fats, the blood pressure,BMI do not have the relevance. [Conclusion]1 In blood serum VEGF,the SICAM-1 level has the varying degreein the differenttime DN patient advancing, prompts VEGF and SICAM-1 in the DN occurrence development the vital role, and has the possibility to become monitor soneof DN condition change effective targets.2 In diabetes patient,blood serum VEGF and SIC AM-1 presents the correlation, they also present the correlational dependence with UAER, prompts VEGF to be possible to induce the SIC AM-1 expression, lies between leads the white blood cell and the endothelial cell attaches suddenly, causes the endothelial cell damage, the extracellular matrix to pile up,blood vessel insight increase, this process and DN formation close correlation intercellular space adherency member -1,Urine albumin excretion rate.