The Effects Of Cxw On The Level Of SOD And MDA In Rats With Bleomycin-induced Pulmonary Fibrosis

Objective: To observe the effect of Chuan-Xiao-Rong-Ye (CXW) on the level of SOD and MDA in rats with Bleomycin-induced pulmonary fibrosis. Method: Ninety male Wistar rats in good conditions were divided into three groups at random: control group (NS group n=30), model group (BLM group n=30). treatment group (CXW group n=30). Rat pulmonary fibrosis was induced by intraperitoneal injection of bleomycin A₅ (15mg/kg body weight) for ten days. On the 1th, 14th, 28th day after administration, ten rats of each group were sacrificed respectively. On the 28th day, the lungs were harvested for histopatholo-gical examination, and the width of alveolar septa and pleura depth were measured. At the same time, the lung coefficients and the hydroxyproline (HYP) of lung tissues hemogenate were detected. On the lth, 14th, 28th day, the contents of superoxide dismutase (SOD) and malonyldialdehyde (MDA) were detected respectively, and the changes of the number of cells and classification of the bronchoalveolar lavage fluid(BALF) were studied. Results: 1) The width of alveolar septa and pleura depth of BLM group were significantly higher than those NS group on the 28th day (P< 0.05), and so were the lung coefficients and levels of HYP in lung tissues hemogenate of the two groups (P<0.05). Those of the CXW group increased at some extent (P<0.05), but they were not significanter than BLM group (P<0.05); 2) Compared with those of the NS group, the percentage of inflammatory cells in BALF and the contents of MDA in BALF and lung tissues hemogenate of the BLM group increased significantly on the lth, 14th days, and the contents of SOD in BALF andlung tissues hemogenate of the BLM group decreased significantly on the lth day (P<0.05). Those changes in CXW group was in a small extent. Moreover, no significant changes were found in serum levels of SOD and MDA between three groups (/)>0.05). Conclusion: CXW may increases content of SOD, and decreases content of MDA. At the same time, it may inhibit collagen deposition in some extent and protect pulmonary tissues.