Effect Of VMIP-Ⅱ On TCRVβ Gene Expression And T Cell Clonal Expansion In SIV Infected Macaca Fascicularis

Objective: vMIP-Ⅱ was an analog of chemokine ,which had a broad-spectrum antagonism to other chemokine receptors.It can prevent HIV to enter immune cells and enhance the immune system of protecting main effect immune cells. We explored the T cell receptor β chain variable region (TCRVβ) repertoire perturbations and T cell clonal expasion in Macaca Fascicularis infected with SIVmac251. Three groups were involved to evaluate whether initiation of vMIP- Ⅱ therapy during primary infection influences the dynamics of T cell mediated immune responses. Methods : The TCRVβ repertoire was analyzed by semiquantitative polymerase chain reaction in PBMC samples obtained form 11 Macaca Fascicularis preinfection and 17 days post infection. Then use high sensitive gene scan techniques to identify the clonality of specific Vβ7 subfamily which demonstrated an expansion for SIV. Results : All 14 TCRVβ subfamilies were detected in nomal samples,the amounts of subfamilies was not changed post infection. Vβ7,8,14,16 subfamilies were expanded in some Macaca Fascicularis in group SIV and vMIP-Ⅱ ,which was more intensive in the latter. The group which had ART therapy also showed an increase in Vβ7,8 subfamilies. The gene scan analysis told us quality of T cell expansion in dominant VP7 expression turned from polyclonal to oligclonal in 12 samples of Macaca Fascicularis.Conclusions : Some specific Vβ families are clonal expanded in SIV primary infected Macaca Fascicularis, and this expansions maybe virus specific. vMIP-Ⅱ may enhance this kind of increase of having higher quantity . vMIP- Ⅱ therapy during primary infection influences the dynamics of T cell mediated immune responses,mainly induce the expansions of immune cells and strength the fuction of immune system against infection.