| Objective :Through determinatin of pesticides residues and heavy metal in the famous Chinese Traditional Patent medicine of our province, situations of the pesticides residues and content of heavy metal in the Chinese Traditional Patent medicine of our province are understood,which may offer scientific basis for the fact that quality control in production and security of use medicine in clinical and offer scientific reference basis to export Chinese Traditional medicine and achieve Chinese Traditional medicine modernization for our province.Method and result: First part,Determinatin of pesticides residues of organic chlorine in the traditional Chinese Patent medicine in Fujian.,Chinese Traditional Patent medicine were selected from Fuzhou , Xiamen , ZhangZhou , Jianyang ,etc. the sample is drawn through the the mixed solvents of acetone and distilled water by supersound, extract with Methylenechloride, add sodium chloride makes organic and water divide layer, purified by concentrated sulfuric acid, centrifugated, take upper clear liquid concentrated , fixed volume. separated by unite ov-1701 quartz capillary chromatogram The average recovery rate 83. 01%-97. 72%,RSD: 2. 94%- 6. 17%. The content of organic chlorine pesticide is lower accord with the national standards.Second part: determination of Organophorous pesticide residue in Chinese Traditional Patent medicine in Fujian.the sample are draweded through the mixed solvents of acetone and distilled water in the condition of constant temperature by vibrator, purge sample with silica gel columella by gradient elution adsorbent system of normal hexane and acetone, acetic ester, the mixed solvent of acetic ester and acetone, the mixed solvent of acetone and methanol,converged eluting liquid , concentrated in water bath close to dry by rotary evaporimeter, hold it to 1ml definitely. separated by HP-1701 quartz capillary column, detected by NPD . The recoveries were 80.1%~95.3% with RSD 2.5%~6.6%, The range of detectable limits were 5.21×10~-3~3.26×10~-2 ng .dichlovos, Methamidophos, Acephate and theother eight kinds of pesticides aren't found in the sample examined.Third part:, Determination of Heavy Metal in Chinese Traditional Patent medicine in Fujian, the sample accurately weighed was placed in crucible, carbonizing on zoom type electric hot plate with little current to no smoking, then migrate to massmann furnace ashing; the ash was dissolved in 0.5 nitric acid, solution was migrated to 10-25ml measuring flask, Washing crucible repeatedly, amalgamating washing liquor to measuring flask and fixed capacity to scale, mixing evenly ,the lead and cadmium are detected by atomic absorption spectrometers. 1.00-5.00g sample weighed was placed to conical flask, added 45ml nitric acid and 10ml sulphuric acid, heated refluxing 2hour, colded. the solution is filted to measuring flask, washing conical flask, filter, washing liquor was converged to measuring flask, adding the water to scale, mixing evenly, mercury is detected by Cold atomic fluorescence spectrophotometer parameter; the 1.00-5.00g sample was placed into beaker, adding 10ml nitric acid, mixed thoroughly ,added 5ml sulphuric acid, left to stand for 24h; the next day ,added 2.5ml perchloric acid, heated at low temperature, add 10ml mixing solution of 5% ascorbic acid and 5% thiocarbamide, translated to measuring flask. Arsenium is detected by atomic fluorescence spectrophotometer parameter, the percentage of theHeavy Metal in the sample detected in this experiment: the lead is 0, cadmium is 0 ,mercury is 53.19% and Arsenium is 2.13%.Conclusion Organochlorine pesticides is low in the sample examined, indicating Chinese Traditional Patent medicine in our province organic chlorine polluted lightly by organochlorine pesticides, no Organophorous pesticide are found the sample,Organic chlorine , organic phosphorus agriculture chemical have a little effect on health in some Chinese Traditional Patent medicine of our province, mercury and arsenic content exceed standard in some Chinese Traditional Patent medicine, it must take measures further to reduce content. Guarantee that it is healthy to use medicine.
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