| Anthocyanins, a water-soluble pigment, widely exist in flower, fruit and stem of plants and bring many colour of most plant, such as blue, red, purple and yellow. Its stucture consists of anthocyanidin and saccharide through che mical bond between them. It has strong dyeing ability and many physical functions, such as protecting skin, cutting off blood-fat, dieting, restraining cancer, delaying senescence and improving eyesight. It can be used in many field, such as food, drug and cosmetic. Investigation and research were carried out for several methods of extracting anthocyanins and comparing these methods, purifying anthocyanins through thin layer chromatography and column chromatography and finding out optimum chromatography material and eluting solvent, and ability of reducing blood-fat.Investigation were carried out for several methods of extracting anthocyanin, such as water extracting, ultrasonic extracting, enzyme extracting and super critical fluid-enzyme extract ing. There is no report so far for method of super critical fluid-enzyme extracting. The result showed that extracting rate of water extracting was the lowest 2.7% and that of super critical fluid-enzyme extracting was the highest 6.5% through comparing these extracting methods. Through orthogonal experiment analysis, the optimum condition of enzyme extracting, tempera ture 30℃, pH value 7.0, solid-to-liquid ratio 1:6, enzyme consumption 24IU, time 90min, was found out and its extracting rate was 6.4%.The result of reducing blood-fat showed that cleaning hydroxyl free radical rate of anthocyanin was 38.8% when concentration of anthocyanin was 0.4mg/mL, and restraining lipid dioxition rate of anthocyanin was 13.4% when concentration of anthocyanin was 0.4mg/mL.The high fat model of big white mouse was established. The result showed that cutting cholesterol rate was 22.34% when anthocyanin dosage was 0.5g/d, cutting triglyceride rate was 34.4% when anthocyanin dosage was 0.6g/d.
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