Ex Extraction, Purification And Initial Pharmacokinetics Of Shengmai Tablet Main Effective Component

Objective ShengmaiYin is a compound preparation of Red ginseng,Ophiopogon, Schisandra composition, with Yiqi Fumai, Yangyin ShengJineffectiveness, efficacy, and commonly used in clinical medicine. It is nowreplaced by tablet formulations, based on the study of literature shengmai tabletextraction process, purification process, pharmacokinetic studies wereinvestigated, and the establishment of the UV levels measured and highperformance liquid measurement method.Methods In the extraction process of research, this paper is mainly based on theactive ingredient of shengmai tablet as ginsenosides, ophiopogonin,schisandralignans. And investigate the UV absorption spectrum to ginsenosides, Schisandralignans.Take ginsenosides, Schisandra lignans and the rate of extractum to beindex,and take extraction time, extraction times, extraction solvent solubility,extraction solvent multiples as the factors.At last,ultimately determine the besttechnology for the use of8times the amount of70%ethanol extracted three times,each time best two hours to extract the results, and content extraction rate than theofficial method, the extraction time is shortened.In the purification process, based on the extract process, take ginseng totalsaponins, lignans content as the reference indicators and adsorptioncapacity,desorption rate as factors to preferred five kinds of macroporous resins,and ultimately determine the best technology D-101to be the best. Then leakcurves were investigated, sample concentration, sample rate, the level of cross-volume, elution concentration, elution rate, elution volume and other factors, ultimately determine the best process for the concentration0.16g/mL to sample9BV the2BV/h rate, then with water after4BV/h balance6BV, with70%ethanol4BV/h speed eluted6BV.In the study of the pharmacokinetics, first determine the method weredetermined by HPLC method ginsenosides Rg1, ginsenoside Re, Schizandrin thenstudied compound compatibility changes shengmai compound, and the resultsexpressed in its extraction and compound purification project with varying degreesof interaction, and therefore determine the pharmacokinetic study in researchmethods on behalf of, the compound is to be administered, and determination. Inspecific pharmacokinetic experiments first selected response higher Schisandrol50mg/kg, be administered using the method tail blood to investigate the proteinprecipitation with acetonitrile and ethyl acetate method, using HPLC todeterminate the concentration of blood, and take a preliminary study.Results In the extraction process of research,the best technology for the use of8times the amount of70%ethanol extracted three times, each time best two hoursto extract the results. In the purification process, the best process for theconcentration0.16g/mL to sample9BV the2BV/h rate, then with water after4BV/h balance6BV, with70%ethanol4BV/h speed eluted6BV. In the study ofthe pharmacokinetics,we make the methord.Conclusion This study provides a scientific basis for shengmaiyin of dosageform reform of shengmai tablets.