Separation And Identification Of Candida Dubliniensis With PCR-RFLP Method In Genital Candidiasis

Candida albicans is generally acknowledged to be the most pathogenic Candida species. However, over the past decade, the incidence of infections caused by C.albicans has decreased. Relatively, the incidence of infections caused by other Candida species has increased. The recent emergence of Candida dubliniensis as a new Candida specie coincide with this epidemiological shift.Candida dubliniensis ,as an opportunistic pathogen ,has been recovered primarily from the oral cavities of HIV-infected individuals and AIDS patients in Dubin 1995.While,more recent papers have established C.dubliniensis in HIV-negative patients. The widespread distribution of C.dubliniensis throughout the world warrants in-depth investigation, particularly because resistance to the commonly used antifungal drug fluconazole has been found in clinical isolates and because fluconazole-susceptible isolates have been shown to be able to rapidly develop resistance to the drug in vitro.It is essential to be able to identify C.dubliniensis. While C.dubliniensis is closely related to C.albicans,and its phenotypic and genotypic characteristics is similar to that of C.dubliniensis. Our research objective is to provide a PCR-RFLP method for unequivocal differentiation of C.dubliniensis from C.albicans and investigate the prevalence of C.dubliniensis among the clinical Candida isolates in Tianjin General Hospital.All 400 clinical isolates are sampled from patients in the dermatology department of Tianjin General Hospital.One standard isolates of C.albicans(ATCC-11006)and one standard isolates ofC.dublinniensis(CBS-8501)are presented by Beijing Medical University.All isolates can produce germ tube after 2h of incubation in serum. Following 48h of growth at 37℃ on CHROM agar ,their colonies appear blue-green color. The isolates which appear blue-green color on CHROM agar then are investigated...