| Berberis L. belongs to the wilding medicinal plants that have a wide range of application, in common use because it distributes extensive, and its supply is abundant. Many categories can be the substitute usage of the in common use Chinese herbal medicine, the great majority category all contain various alkaloid,such as Berberine, Berbamine, Jatrorrhzine, Paimatine,and they have various physiology activities. Berberis heteropoda in Xinjiang distribute widely, and the resource is abundant.It is a kind of good wild resources plant and has great potential to be developed. Berberis heteropoda Schrenk belongs to Berberis L. of Berberidaceae.This experiment was divided into the four-part to carry on qualitative and quantitative analysis for the four alkaloids (Berberine, Berbamine, Jatrorrhzine, Paimatine) within the fruit,the leaves,the stems and the cauline teguments of blackfruit barberry. Then the more two alkaloids (Berberine, Berbamine) of them were distilled and separated. At last,we compared the antimicrobial activity between Berberine and Berbamine. Result is as follows: 1.This research made qualitative analysis which made use of chemical reaction and thinlayer chromatography(TLC) for Berberine, Berbamine, Jatrorrhzine and Paimatine. The result shows that the fruit and leaves of blackfruit barberry imply Berberine and Berbamine,the stems and the cauline teguments of it imply Berberine, Berbamine, Jatrorrhzine and Paimatine. 2.HPLC(high efficiency liquid chromatography) was applied to determine the content.The result is: It contains 0.032% of Jatrorrhzine and 0.002%of Paimatine in the fruit; 0.056% of Jatrorrhzine and 0.011% of Paimatine in the leaves; 0.256% of Berberine, 0.29% of Berbamine, 0.045% of Jatrorrhzine and 0.008% of Paimatine in the stems;0.377% of Berberine,0.412% of Berbamine, 0.115% of Jatrorrhzine and 0.009% of Paimatine in the cauline teguments. 3.Selected the branches of blackfruit barberry to experiment. First, subjected Berberine and Berbamine to 0.5%～0.8% of dilute vitriol. Second ,made use of their different solubility to separate them.Chose aether to sublimate Berberine in the end.Used repetitiously acid dissolving and alkali depositing to sublimate Berbamine. The weight of Berberine crystal was 196.5mg,and the one of Berbamine crystal was 308.2mg.At last,measured their melting point and infrared absorption spectrum to identify the crystals.The result shows that these crystals are Berberine and Berbamine,and their purity is better. 4.We used the crystals of Berberine and Berbamine as materials to compare the antibacterial activity between Berberine and Berbamine. As a result, Berberine had greater antimicrobe activity to Staphylo-coccus aureus ( MIC:0.02mg/mL) and Bacillus subitilis (MIC: 0.06mg/mL) than E.coli(MIC:0.4mg/mL) and yeast(MIC:0.7mg/mL). Berbamine had greater antimicrobe activity to E.coli(MIC:0.3mg/mL) than Staphylococcus aureus(MIC:1mg/mL), Bacillus subitilis(MIC: 1mg/mL) and yeast(MIC:0.8mg/mL). |
