Evaluation About Air Contamination In The Sickrooms Of SARS Patients, Basic Research About SARS Air-Isolation Strain And Foundation Of Relative Virus Database

SARS-CoV is a kind of novel single RNA virus which can arose zoonosis. This kind of virus can spread quickly via short-haul airborne infection and hand-in-hand infection. In hospital and at home SARS-CoV takes on prominent assembly phenomena. Admittedly airborne infection can be interfered by multi-fold factors, the most important factor is livability of SARS-CoV in the aerosol state, and the others include temperature and humidity of surroundings, ultraviolet radiation, and some kinds of chemic contamination.By the way of RT-PCR and cell culture of air samples from two hospitals we can offer effective advise about controlling SARS-CoV airborne infection. We used Andersen microbial sampler 2 stage (AMS-2) to sample incessantly at five spots (Corridors, Exhaust fans, cleaning-districts, sickrooms and sickroom-balcony) and obtained 112 air samples in different times. Then we accomplished washing of air samples, isolation of total RNA, RT-PCR, Vero-E6 cell culture and gene-sequence analysis. We have successfully separated active SARS-CoV (called LK309 strain) from air samples and SARS-CoV RNA was detected at all five spots in two hospitals, among which the detecting positive rate is 25 (52. 1%) of 48 air samples from sickrooms in XiaoTang-hill hospital and is 9 (29. 03%) of 31 ones from sickrooms in PLA 309 hospital, the results showed that convalescence patients still exhale viruses by respiratory tract and at 5 to 10m distance...