Study On The Toxicity And Pharmacological Effects Of The Capsule Of Microbial Grease PUFAs Microcapsule Powder

To verify whether the capsule of microbial grease PUFAS microcapsule powder have immune regulation function and safety,we carry on pharmacology and toxicology trial for the drug.In the pharmacology trial,administrating via oral,give three groups of KunMing mouse 0.45,0.9,1.40 g/kg.bw three doses of the drug, we (1) test body weight,immune organ/body weight ratio. (2) adopt the method of MTT to carry on lymphocyte multiplication induced by ConA assay.(3) adopt the method of ear swell to carry on the delayed type hypersensitivity induced by DNFB.(4) adopt the method of modified Jerne's method to carry on Haemolytic plaque assay(HPA) assay.(5) adopt the method of blood concretion method to check level of hemagglutinin in serum.(6) adopt the method of in-half body to examine the macrophages of the abdominal cavity of phagocytose to the erythrocytes assay. (7) adopt the method of injecting Indian ink in tail vein.to test the ability of mouse carbon release.The results show:compare the control group,(1)the drug have no significant effects for body weight and immue organism/weight body ratio(P > 0.05).(2)middl,especially high dose can promote significantly the lymphocyte multiplication ( P<0.05, P<0.01,high dose group's average relative OD is 0.041,control's is 0.031) (3)middl,especially high dose can agaravate significantly the delayed type hypersensitivity induced by ConA(P<0.05, P<0.01,high dose group's average relative weight of left ear to right ear is24.30,the control's is 20.13) .(4) middl,especially high dose can aprove significantly antibody producing capacity(P<0.05, P <0.01, high dose group's average haemolytic plaque numbers is 387,the control's is 319) .(5) middl,especially high dose can enhance significantly the level of hemagglutinin in serum(P< 0.05, P<0.01, high dose group's average HC₅₀ is 77.4,the control's is 45.2) .(6) middl,especially high dose can strenghthen significantly the ability of macrophagous of the abdominal cavity of phagocytose to the erythrocytes (P<0.05, P<0.01, high dose group's average phagocytic percentage is34.5, phagocytic index is 0.314, the control's is27.4 and0.319 ) .(7) middl,especially high dose can improve significantly carbon release capacity(P< 0.05, P<0.01, high dose group's average carbon phagocytic index is 6.047,the control's is 5.372).So,we can conclude the drug can regulate immune function of mice.In the toxicological trial,according to ((food safety toxicology evaluatve procedures and methods)) published by Chinese hygiene department,we carry on (l)mouse acute to toxicity assay,(2)genetics toxicity trial(Ames assay, mouse bone marrow polychromatic erythroblasts micronuleus assay,mouse sperm abnormality assay),(3)feeding Wistar rat the drug for 30 days assay.Result:(l)after administrating via stomach for 2 weeks at the dose of 13.2g/kg.bw,all mice are alive and healthy,(2)the results of Ames test to salmonnell typhimurium TA97,TA98,TA100,TA102 were negative whatever using S-9 or not.the results of mouse bone marrow polychromatic erythroblasts micronuleus assay and mouse sperm abnormality assay were negative,too.so,it's showed that no genetics toxicity had been seen in those mice.(3)After feeding the drugs there is no obvious change of rat the drugs for 30 days,there is no obvious change on clinical behave.blood compositions,biochemical reaction,pathological change.So,we conclude the drug is safe.We have complished the study of the toxicity and pharmacological effects of the capsule of microbial grease PUFAS microcapsule powder and found the drug is safe and have immune effects.So,my study give assay evidences to develop the drug to factional health-preservation product.