| DNA vaccine, as a new approach of immunity, being used for preventionand cure the infection of HSK, and clearing up recurrent infection followingreactivation from latency. has drawn the attention of people gradually, DNAvaccine is target for both humoral and cell mediated immunity,,and make it easyto construction of multi-valent DNA vaccine , This research has paved the wayfor study on truncated HSV-1 gB gene DNA vaccine , and also make the foundfor the const ruction of multi-valent DNA vaccine of HSV-1.The glycoprotein B is attractive choices for subunit vaccines because theyare targets for both humoral and cell mediated immunity, gB also has a highdegree of identity comparing HSV-1 and HSV-2, therefore, may provideprotection against both HSV-1 and HSV-2. Further, there is an even higherdegree of conservation between strains of the same virus type. Glycoprotein Bis also a potent immunogen capable of inducing both neutralizing antibody andvirus-specific cytotoxic T cells.The gB structural gene sequence encodes 904amion acids,it contains a 30-residue N-terminal signal sequence ,a 697-residueexternal domain,a 68-residue transmembrance domain and a 109-residuecytoplasmic domain. External domain were report that epitopes of potentneutralizing antibodies cluster in three major antigenic domains , D1,D2,D5a,Itcontaims fourteen neutralizing antibodies cluster contain ,seven antigenic site arecomplement dependent and eight antigenic site are neutralization antibodydomains. The immunodominant epitope of cytotoxic T lymphocytes is derivedfrom 468-478aa.Therefor, external domain sequence ,as a ideal antigen gene willbe used to construction DNA vaccine of HSV-1.By means of construction DNA vaccine is eukaryotic expression vectorpcDNA3 ,which was made of three portion , â‘ Pcmv root in humancytomegalovirus contributing to express backward positionâ‘¡polA root inbovine growth hormonegene â‘¢the E.coli root of coli form, to the effect that...
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