The Effect Of Ionizing Irradiation On Toll Signaling In Macrophages Of Mice

The organic immunity can be generally categorized into adaptive immunity and innate immunity. Organism recognizes determinants of microbe by the elements in the system of innate immunity when humoral and cellular immunity start in the early stage, then the microbe innate immunity is regulated and the adaptive immunity is built up. The process of immune response depends on the interactions of immune cells of the immune system, including direct contacts of immune cells and cellular factors or mediums releasing. Immune cell membrane molecules including antigens, receptors and other molecules on the cell surface are the bases of recognition between immune cells or between cells and mediums. Ionizing irradiation can stimulate the organism's immune responses and biological effects by cell signal transmissions. Toll signal is the organic innate immunity pathway, i.e.,receptors of CD14,TLR4/TLRs on the membrane→adaptor protein MyD88 in the cell→nuclear transcription factor κB→effector cytokine IL-18. We observe the changes of several important parameters including CD14,TLR4,MyD88 and IL-18 after ionizing irradiation. There are no reports on the effects of TLRs signal pathway of radiative reaction at present. The experiment is a unique research in a creative way, it fills up the blank of this field. Objective: To explore the influence and mechanism of ionizing irradiation on TLRs-mediated signaling pathway of organic immune cells. To provide theoretical foundations on clinical irradiation therapy of malignant spine tumors. Materials and methods: Collecting peritoneal macrophages of mice which have been radiated on the whole body with high doses radiation (HDR) and low doses radiation (LDR) by the X-ray machine. Culturing the collections with the culture medium of RPMI1640 in 37℃, 5%CO2 culture box. Observing the time-course changes and dose-effect changes of CD14, TLR4 and MyD88 by Flow cytometry (FCM). ELISA was used to detect the time-course changes and dose-effect changes of IL-18 in Toll signal pathway in the peritoneal macrophages of mice. Results: 1,The expression of CD14 and TLR4 in peritoneal macrophages of mice after whole body irradiation(WBI): (1) Time-course changes: both of them begin to increase at 4 h,to reach the peak between 8h and 16h,then drop gradually to sham-irradiation level at 48h; (2) Dose-effect changes:the expressions are up-regulated. The statistic significances are achieved from 0.075Gy, and the peak emerges between 1.0Gy and 2.0Gy. Then it drops between 4.0Gy and 6.0Gy. The changes'trend are same for both receptors. 2,The expressions of MyD88 in peritoneal macrophages of mice after WBI: (1) Time-course changes: both HDR and LDR increase the expressions of MyD88. The peak emerges between 16h and 24h,and...