Protective Effects Of Topamax In Neonate Rats With Hypoxic-Ischemic Brain Damaged

Hypoxic-ischemic encephalopathy(HIE) newborn is an injury ofdisease of nervous system, which is caused by differenthypoxic-ischemic (HI) factors in perinatal period and shows highmortality rate. Most of patients with HIE accompany sequelae such asmental retardation\ cerebral palsy\epilepsy. HIE is common reason toresult in nervous system invalid of children and become investigativefocus. pathogenesy of HIE is extremely complex and mechanism ofbrain injury is unclear.Most study of HIE pathogenesy is focus on neurone. Accompanywith development of research on colloid cells, Scholors observed thatcolloid cell is very active after HI and emerge multiple molecular levelchanges., which has two way actions to neurone damaged. Colloidcells show various plasticity and reaction to microinviroment indifferent development stage. Colloid cells of newborns (rats) possessbetter plasticity than adults` (rats`). Nevertheless, The former is moresusceptibility to HI. Previous research on colloid cells main localizemajority rats and seldomly study on newborn rats.Topamax is a new broad-spectrum anti-epileptic drug which hasdistinctive structure. Recently, abord scholors had observed thattopamax possess potential neuroprotective effect. Otherwise the studyon influent of topamax to horizontal cell change after HIBD andneurocyte apoptosis is seldom.Thus, we select term-fetation rats whichboth uterine artery are deligated 25minuates and make them intoHIBD models. We apply ntragastric administration methods toobserve rats` brain tissue moisture capacity\colloid cell changes\neurocyte apoptosis and learning-memory capacity with Morris watermaze test.ObjectiveTo explore Protective Effects of Topamax in Neonate rats withHypoxic-Ischemic Brain Damaged.methodsThe rats are made into HIBD models and divided into threegroups,which is HI groups and topamax groups of administeringdrug (administering drug one time, administering drug five time). Inthe same time, we set up normal control. Then, we determine eachgroup rats` brain tissue moisture capacity\exhibit cornu ammonis ASTcell changes by immunochemistry staining methods and detectapoptosis cell change with TUNEL methods. Moreover, observe thecapacity of learning and memory of rat by Morris water labyrinth test.Results:1. Each group rats` brain tissue moisture capacity isundistinction within 6 hours.At 12h, group of administering topamaxone time is lower than HI group, but hasn`t difference in contrast tonormal control. group of administering topamax one time and HIgroup at the same level, but is higher than normal control after24h.48h later, group of administering topamax five time is higher thannormal control but lower than the other groups.When 72h passed, rats`brain tissue moisture capacity high to low order is HI group\ group ofadministering topamax one time\ group of administering topamax fivetime\normal control.At 7th day, each group is equal.2. AST cell of brain tissue changed: (1)rats cornu ammonisGFAP positive cell density contrast: 3h later,normal control didn`texpress GFAP positive cell, group of administering topamax one timeis lower than HI group,which is higher than normal control After6h.After 24h past, HI group degraded to normal control level andlower than group of administering topamax one time.3 days later,normal control is the lowest and other groups are equal. At 7th day,group of administering topamax one and five time emerge nodefference,but lower than HI group and higher than control group. At14th day, group of administering topamax five time is lower than HIgroup and group of administering topamax one time and is higher thannormal control. 21 days later, HI group is the highest and normalcontrol is the lowest. At 28th day, group of administering topamaxone and five time are at the same level, but are lower than HI groupand higher than normal control. (2) rats cornu ammonis GFAP positivecell gray scale comparison: 3h later,normal control didn`t expressGFAP positive cell, group of administering topamax one time ishigher than HI group,which is lower than normal control After 6h.After 24h past, group of administering topamax one time is same asHI group and lower than normal control.3th and 7th day, normalcontrol is the highest and other groups are equal. 14d\21d\28d later,each group is equal.3. Apoptosis cell change; 3h and 24h later, group of...