Study Of Antiulcer Activities Of Total Alkaloids From Rhizoma Coptis Chinensis On The Experimental Gastric Ulcers

Objective Study of the effects and mechanisms of TA on the experimental gastric ulcers.Methods (1) The antiulcer effects of TA were evaluated using 4 experimental gastric ulcers that were established by water-immersion stress, intragastric ethanol, acetic acid erosion, and pylorus ligation, respectively. (2) The effects of TA administrated previously orally on gastric secretion in rats by pylorus ligation were observed, and compared with that of TA administrated into dodecadactylon. (3) Using pathomorphologytechnique, the protective effects of TA on gastric ulcers induced by ethanol in rats were further observed. Furtheremore, the contents of nitric oxide (NO), malondialdehyde (MDA), reactive oxide ( . OH), and superoxide dismutase (SOD) activity from rats gastric mucosa were measured to explore the interrelation between therapeutic effects of TA and these factors. (4) The effects of TA on the expressions of nervous nitrogen monoxide synthase (nNOS), endothelial NOS (eNOS), and induced NOS (iNOS) in rats gastric mucosa damaged by ethanol were analysed using immunohistochemical method.Results (1) TA significantly inhibited ulcerative formationinduced by both water-immersion stress and pylorus ligation, obviously antagonized the mucosa damages induced by ethanol, in dose-dependent manner, and also accelerated healing of the gastric ulcers eroded by acetic acid. The antiulcer activity of TA was superior to that of Ber and...